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白腐菌trameteshirsute对六氯苯的降解及其条件优化

DOI: 10.3724/SP.J.1145.2009.00698, PP. 698-702

Keywords: 白腐菌,响应面法,六氯苯,生物降解

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Abstract:

在液体体系下,筛选了5株白腐菌,对六氯苯进行了降解研究,并优化了白腐菌trameteshirsuteth对六氯苯的降解条件.结果表明,不同白腐菌均能降解六氯苯,其中白腐菌trametessp.tr对六氯苯降解率最高,达90.21%;而白腐菌t.hirsuteth对六氯苯降解率为87.08%,但生物量最高,达3.33g/l.通过响应面法优化白腐菌t.hirsuteth降解六氯苯的条件,结果显示,转速、接种量和培养时间是影响六氯苯降解的主要因素.优化后的最佳条件为:转速125r/min,菌丝接种量8%(v/v),培养时间2d,温度28℃,ph为7.在优化条件下,2d内白腐菌t.hirsuteth对浓度为10mg/l的六氯苯降解率和降解量分别可达91.52%和2.288mgl-1d-1.图2表7参17

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