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OALib Journal期刊
ISSN: 2333-9721
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抗柑桔溃疡病菌可溶性单链抗体的表达及鉴定
DOI: 10.3724/SP.J.1145.2009.00626, PP. 626-629
Keywords: 柑桔溃疡病菌,单链抗体,可溶性表达,抗原结合活性
Abstract:
前期采用核糖体展示技术筛选了抗柑桔溃疡病菌（xanthomonasaxonopodispv.citri,xac）高亲和力单链抗体（singlechainvariablefragment,scfv）gx13、gx44和gx95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌hb2151中进行可溶性表达，点印迹和western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法（elisa）检测纯化单链抗体的抗原结合活性.结果显示，3株抗柑桔溃疡病菌可溶性单链抗体在大肠杆菌hb2151中均获得成功表达，表达产物分子量（mr）约为32.0×103，主要集中于细菌周质腔中.elisa检测纯化的单链抗体都具备抗原结合活性，可进一步应用于柑桔溃疡病菌的免疫诊断和病害防治.图4参14
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