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OALib Journal期刊
ISSN: 2333-9721
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用ssr标记检测杂交籼稻三系亲本的遗传差异
DOI: 10.3724/SP.J.1145.2009.00585, PP. 585-590
Keywords: ssr,籼稻亲本,遗传多样性,聚类分析
Abstract:
随机选用分布于水稻（oryzasatival.）12条染色体上的110对引物，共筛选出26对具有多态性的ssr引物，对24份杂交籼稻三系亲本材料进行遗传多样性和亲缘关系分析.全部24份亲本中共检测出116对等位基因，每个ssr引物可检测到的等位基因数目为2~7个不等，平均每对引物可以检测到4.4个等位基因.多态信息量（pic）的变动范围为0.0689~0.8036，平均pic值为0.6034；期望杂合度（he）的变动范围为0.0816~0.8298，平均值为0.6169；多样性指数（i）变幅为0.1732~1.7913，平均为1.1600.对供试亲本材料根据遗传距离进行upgma聚类分析，结果表明，ssr微卫星标记可以将24份亲本材料分为3个大群，各组亲本间的遗传差异较大.通过分子标记辅助育种,可以有效地鉴定供试亲本材料之间的亲缘关系，分析预测新种质材料的遗传差异，可作为选育强优势组合杂交水稻的重要参考指标之一.图2表3参24
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