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OALib Journal期刊
ISSN: 2333-9721
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紫花苜蓿根际氢氧化细菌的分离与鉴定
DOI: 10.3724/SP.J.1145.2009.00650, PP. 650-654
Keywords: 紫花苜蓿,氢氧化细菌,生理生化特性,16srdna,系统发育树,假单胞菌属
Abstract:
利用气体循环培养体系从陕西乾县hup―豆科植物紫花苜蓿（medicagosativa）根际土壤中分离获得37株细菌.菌株氧化氢能力测定结果表明，8株菌氧化氢和自养生长能力较强，初步确定为氢氧化细菌类群；根据其形态特征、培养特征和生理生化特性，鉴定为7个不同属：假单胞菌属（pseudomonas）、邻单胞菌属（plesiomonas）、脂肪杆菌属（pimelobacter）、黄色杆菌属（xanthobacter）、勒米诺氏菌属（leminorella）、地杆菌属（terrabacter）和稀有杆菌属（rarobacter）；其中氧化氢能力最强的优势菌株wmq-716srdna序列（genbank登录号为eu807744）长度为1451bp，gc含量为53.8%，其核苷酸序列与假单胞菌属同源性高于99%，在系统发育树上位于同一分支，将wmq-7菌株鉴定为假单胞菌属（pseudomonas）.图2表5参16
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