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OALib Journal期刊
ISSN: 2333-9721
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不同宿主植物根瘤frankia及其生物学特性
DOI: 10.3724/SP.J.1145.2009.00645, PP. 645-649
Keywords: frankia菌,生物学特性,交叉侵染,宿主特异性
Abstract:
对从木麻黄、杨梅、桤木和胡颓子等宿主植物根瘤中获得的19株分离菌的离体培养形态特征、生理特性和交叉侵染特性等进行了比较分析.结果表明，各分离菌均有frankia属所特有的分枝状菌丝、孢囊、泡囊或串珠状菌丝等形态结构；细胞壁类型多属胞壁ⅲ型，生理类型多属b型，无氮诱导培养下都具有固氮酶活性，在bap、ja或s培养液中菌体生长较好，以吐温-80和酪蛋白水解物为最佳碳、氮源.但不同宿主分离菌的形态和培养特征差异明显，木麻黄属分离菌菌丝较粗，孢囊数量较少，在bap培养液中多呈荔肉白絮状颗粒沉淀；杨梅属菌丝较细，孢囊数量较多，在bap培养液中多是浅红色颗粒沉淀；桤木属和胡颓子属的菌体形态特征在bap培养液中与木麻黄属的相似，但桤木属菌丝较细，胡颓子属的菌丝较粗.根据回接及交叉侵染特性可将分离菌分为2个宿主特异类群：能侵染木麻黄苗木的木麻黄类群；只侵染原宿主并能在杨梅属、胡颓子属和桤木属间相互侵染，但不能使木麻黄属苗木结瘤的杨梅？桤木？胡颓子类群.图2表5参19
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