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OALib Journal期刊
ISSN: 2333-9721
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重组balb/c鼠冷诱导rna结合蛋白cirp多克隆抗体的制备与鉴定
DOI: 10.3724/SP.J.1145.2009.00634, PP. 634-637
Keywords: 鼠,冷诱导rna结合蛋白,多克隆抗体,基因重组
Abstract:
冷诱导rna结合蛋白（cirp）是第一种在哺乳动物细胞中被发现的冷休克蛋白，伴随着细胞冷应激过程过量表达.为进一步研究冷诱导rna结合蛋白cirp的生物学功能，构建重组balb/c鼠cirp的原核表达载体，重组balb/c鼠cirp蛋白经ni-nta亲和树脂纯化后免疫獭兔制备抗cirp多克隆抗体，以间接elisa和westernblot检测抗体效价和抗原特异性.本实验成功构建了cirp的原核表达载体，在e.colixl-1-blue得到了高效表达，利用原核表达包涵体蛋白免疫獭兔获得了高效价的cirp多克隆抗体，该抗体能识别肝脏和睾丸中天然的cirp蛋白.图5参14
| [1] | 2panf,zaratej,choudhurya,rupprechtr,bradleytm.osmoticstressofsalmonstimulatesupregulationofacoldinduciblernabindingprotein(cirp)similartothatmammalsandamphibians.biochimine,2004,86:451~461
|
| [2] | 3yangc,carrierf.theuv-induciblerna-bindingproteina18(a18hnrnp)playsaprotectiveroleinthegenotoxicstressresponse.jbiolchem,2001,276:47277~47284
|
| [3] | 4xuejh,nonoguchik,fukumotom,satot,nishiyamah,higashitsujih,itohk,fujitaj.effectsofischemiaandh2o2onthecoldstressproteincirpexpressioninratneuronalcells.freeradicbiolmed,1999,27:1238~1244
|
| [4] | 5nishiyamah,dannos,kanekoy,itohk,yokoih,fukumotom,okunoh,millanjl,matsudat,yoshidaoandfujitaj.decreasedexpressionofcold-induciblerna-bindingprotein(cirp)inmalegermcellsatelevatedtemperature.amjpathol,1998,152:289~296
|
| [5] | 6nishiyamah,xuejh,satot,fukuyamah,mizunon,houtanit,sugimotot,fujitaj.diurnalchangeofthecoldinduciblerna-bindingprotein(cirp)expressioninmousebrain.biochembiophysrescommun,1998,245:534~538
|
| [6] | 7pengy,yangph,tannerja,huangjd,lim,leehf,xurh,kunghf,linmc.cold-induciblernabindingproteinisrequiredfortheexpressionofadhesionmoleculesandembryoniccellmovementinxenopuslaevis.biochembiophysrescommun,2006,344:416~424
|
| [7] | 14jinfh(金福厚),pangy(庞岩),lisz(李士泽),yanghm(杨焕民),jih(计红),zhaoqx(赵巧香),yinw(尹位).cloningandsequenceanalysisofcoldinduciblerna-bindingproteincdnafromtestistissueinbalb/cmice.chinjapplenvironbiol(应用与环境生物学报),2009,15(1):87~090
|
| [8] | 1nishiyamah,itohk,kanekoy,kishishitam,yoshidao,fujitaj.aglycine-richrna-bindingproteinmediatingcold-induciblesuppressionofmammaliancellgrowth.jcellbiol,1997,137:899~908
|
| [9] | 8hamidaa,mandaim,fujitaj,nanbuk,kariyam,kusakarit,fukuharak,fujiis.expressionofcold-induciblerna-bindingproteininthenormalendometrium,endometrialhyperplasia,andendometrialcarcinoma.intjgynecolpathol,2003,22:240~247
|
| [10] | 9saitot,sugimotok,adachiy,wuq,morikj.cloningandcharacterizationofamphibiancoldinduciblerna-bindingprotein.compbiochemphysiolbbiochemmolbiol,2000,125:237~245
|
| [11] | 10matsumotok,aokik,dohmaen,takiok,tsujimotom.cirp2,amajorcytoplasmicrna-bindingproteininxenopusoocytes.nucleicacidsres,2000,28(23):4689~4697
|
| [12] | 11leeuwfd,zhangt,wauquierc,huezg,kruysv,gueydanc.thecold-induciblerna-bindingproteinmigratesfromthenucleustocytoplasmicstressgranulesbyamethylation-dependentmechanismandactsasatranslationalrepressor.expcellres,2007,313:4130~4144
|
| [13] | 12robinows,camposar,yaok,m,whitek.theelavgeneproductofdrosophila,requiredinneurons,hasthreernpconsensusmotifs.science,1988,242:1570~1572,
|
| [14] | 13pengy,kokkh,xurh,tayd,fungpcw,kunghf,linmcm,kokkh.maternalcoldinduciblernabindingproteinisrequiredforembryonickidneyformationinxenopuslaevis.febslett,2000,482:37~43
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