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OALib Journal期刊
ISSN: 2333-9721
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金鸡菊（coreopsisdrummondii）的抗tmv活性物质
DOI: 10.3724/SP.J.1145.2009.00621, PP. 621-625
Keywords: 金鸡菊,抗tmv活性,1-苯基-1,3,5-三庚炔,活性物质分离
Abstract:
采用活性跟踪法从金鸡菊根中分离获得抗病毒活性物质，经质谱和核磁共振分析，鉴定该物质为1-苯基-1,3,5-三庚炔.采用半叶枯斑法、叶圆盘法测定了该物质对烟草花叶病毒的抑制效果，结果表明，0.2mg/ml的该化合物对tmv表现出较好的体外抑制侵染和增殖活性，其对tmv侵染和复制的抑制率分别为73.5%和84.3%.实时荧光定量pcr测定结果表明，该化合物对tmv外壳蛋白基因的表达有明显的抑制作用，0.2mg/ml的该化合物对tmv外壳蛋白基因表达的抑制率为79.8%.图6表1参19
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