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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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拟南芥tak蛋白激酶的结构预测与功能分析
DOI: 10.3724/SP.J.1145.2009.00610, PP. 610-614
Keywords: tak,磷酸化,lhcii,核心结构域,同源建模,抗体
Abstract:
tak1、tak2和tak3属于tak蛋白激酶家族，由拟南芥核基因编码，其中tak1参与了主要捕光色素复合物lhcⅱ蛋白磷酸化调控与光系统的状态转移.本文使用生物信息学手段对tak1、tak2和tak3蛋白进行了较为系统的分析，发现tak1、tak2和tak3为单次跨膜蛋白，具有保守的激酶活性域、疏水性强的n端跨越类囊体膜、亲水性高的c端处于基质中等特点.在pdb中找到了不少与tak1、tak2和tak3同源性大于30%的蛋白序列，其中大部分也带有酪氨酸激酶保守域，使用同源建模的方法，建立了拟南芥蛋白激酶taks核心结构域的三维结构，围绕蛋白激酶的结构和作用机制关系进行了探讨，设计出多肽抗体，并进行蛋白印迹检测抗体的专一性，为taks蛋白激酶进一步的功能研究奠定了基础.图5表1参15
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