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OALib Journal期刊
ISSN: 2333-9721
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应用高效离子交换色谱分析青枯菌的致病力分化
DOI: 10.3724/SP.J.1145.2009.00713, PP. 713-718
Keywords: 青枯菌,致病力,高效离子交换色谱,激光光散射仪
Abstract:
应用高效离子交换色谱和激光光散射仪检测器对不同致病力的青枯菌进行分析，建立了一种快速检测青枯菌致病力分化的新方法.青枯菌纯培养物经过高效离子交换色谱分离得到3个致病力不同的特征峰，大小依次为峰3组分＞峰2组分＞峰1组分.对10株青枯菌进行色谱分析，并结合番茄组培苗感染试验检测其致病力，结果发现，强致病力菌株经过色谱分离只在峰3的保留时间位置出现单一特征峰，在9d内即可引起100%的番茄组培苗发病；若菌株经过色谱分离形成3个特征峰，则峰3所占的面积比越大，该菌株的致病性相对就越强.25株不同致病力青枯菌的验证试验表明了该方法的可行性，番茄组培苗发病率x与峰3面积比y之间呈现出良好的线性关系，回归方程y=0.9581x+5.4984，相关系数r=0.986.通过对青枯菌色谱行为、致病力、细胞表面黏附的epsⅰ含量三者之间相互关系的进一步研究，发现青枯菌的致病力越强，则细胞表面黏附的epsⅰ越多，峰3所占的面积比就越大.图3表6参15
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