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OALib Journal期刊
ISSN: 2333-9721
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产1,3-丙二醇新型基因工程菌的构建
DOI: 10.3724/SP.J.1145.2009.00708, PP. 708-712
Keywords: 1,3-丙二醇,3-羟基丙醛,重组大肠杆菌,共表达,甘油
Abstract:
以甘油为底物转化生产1,3-丙二醇的生物合成途径中，往往由于还原力nadh的不足，限制了1,3-丙二醇的合成，引起中间代谢产物3-羟基丙醛累积，进而抑制甘油脱水酶的活性，阻碍菌体的生长，严重影响1,3-丙二醇的合成途径.为了解决合成途径中还原力不足这一主要矛盾，本文以大肠杆菌和克雷伯氏菌染色体dna为模板克隆得到yqhd和dhab、dhat基因，构建双启动子表达载体petac-dhab-tac-yqhd及表达载体puc-tac-dhat，成功共转入e.colijm109，得到可利用两种辅酶（nadh、nadph）将甘油转化为1,3-丙二醇且传代稳定的重组大肠杆菌双质粒系统，发酵结果表明，1,3-丙二醇产量提高了28.6%.图6表1参17
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