|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
转座子挽救法克隆鞘氨醇单胞菌cds-1中呋喃丹水解酶相关基因
DOI: 10.3724/SP.J.1145.2009.00677, PP. 677-681
Keywords: sphingomonasagrestiscds-1,呋喃丹,转座子挽救法,基因克隆
Abstract:
用含tn5转座子的自杀性质粒psc123诱变呋喃丹降解菌sphingomonasagrestiscds-1，获得失去呋喃丹降解功能的突变株cds-m1.以pmd18-t为载体在e.colidh5α中构建了cds-m1的基因组文库，采用转座子挽救法对tn5插入位点两侧翼的序列进行克隆与测序，根据测序结果（共4551个碱基）设计引物，从cds-1的基因组中扩增到同样大小的片段，把该片断克隆到广宿主载体ppzp201上，得到重组质粒pcdz1，通过三亲接合的方法把pcdz1导入cds-m1中进行功能互补实验，结果显示cds-m1的呋喃丹水解功能得到了恢复，表明该片断中包含呋喃丹水解酶相关基因.图7表1参14
| [1] | 5paulht,jeffreysk.cloningofacarbofuranhydrolasegenefromachromobactersp.strainwm111anditsexpressioningram-negativebacteria.jbacteriol,1989,171(7):4038~4044
|
| [2] | 6toppe,hansonrs,ringelbergdb,whitedc,wheatcroftr.isolationandcharacterizationofann-methylcarbamateinsecticide-degradingmethylotrophicbacterium.applenvironmicrobiol,1993,59(10):3339~3349
|
| [3] | 10wuj(武俊),xujh(徐剑宏),hongq(洪青),liuz(刘智),zhangxh(张小华),lisp(李顺鹏).isolationofacarbofurandegradingbacterium(cds-1)anditscharacterization.actascicircumst(环境科学学报),2004,24(2):338~342
|
| [4] | 11banhq,arenskotterm,steinbuchela.establishmentoftn5096-basedtransposonmutagenesisingordoniapolyisoprenivorans.applenvironmicrobiol,2005,71(9):5077~5084
|
| [5] | 12sambrookj,fritschef,maniatist.molecularcloning:alaboratorymanual.2nded.newyork:coldspringharbor,1989
|
| [6] | 1scholznk,truelovenk,labeniajs,baldwindh,colliertk.dose-additiveinhibitionofchinooksalmonacetylcholinesteraseactivitybymixturesoforganophosphateandcarbamateinsecticides.environtoxicolchem,2004,25(5):283~290
|
| [7] | 2gianessilp,andersonje.pesticideuseintheuscropproduction:nationalsummaryreport.washingtondc:thenationalcenterforfoodandagriculturalpolicy,1995
|
| [8] | 3begumg.carbofuraninsecticideinducedbiochemicalalterationsinliverandmuscletissuesofthefishclariasbatrachusandrecoveryresponse.aquattoxicol,2004,66(1):83~92
|
| [9] | 4dasac,chakravartya,sukulp,mukherjeed.influenceandpersistenceofphorateandcarbofuraninsecticidesonmicroorganismsinricefield.chemosphere,2003,53(8):1033~1037
|
| [10] | 7fengxh,oult,ograma.cloningandsequenceanalysisofanovelinsertionelementfromplasmidsharboredbythecarbofuran-degradingbacterium,sphingomonassp.cfo6.plasmid,1997,37(3):169~179
|
| [11] | 8goum(苟敏),quyy(曲媛媛),yangh(杨桦),zhoujt(周集体),lia(李昂),guanxy(关晓燕),aiff(艾芳芳).sphingomonassp.:anovelmicrobialresourceforbiodegradationofaromaticcompounds.chinjapplenvironbiol(应用与环境生物学报),2008,14(2):276~282
|
| [12] | 9fengx(冯�),liuxh(刘宪华),songwh(宋文华),daisg(戴树桂).characterizationofsuperiorstrainaebl3ofdegradingcarbamatepesticides.urbanenviron＆urbanecol(城市环境与城市生态),2003,26(6):144~146
|
| [13] | 13fengxh,oult,ograma.plasmid-mediatedmineralizationofcarbofuranbysphingomonassp.straincf06.applenvironmicrobiol,1997,63(4):1332~1337
|
| [14] | 14chaudhrygr,mateena,kaskarb.purificationandbiochemicalcharacterizationofthecarbamatehydrolasefrompseudomonassp.50432.biotechnolapplbiochem,2002,36(1):63~70
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
