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OALib Journal期刊
ISSN: 2333-9721
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过量表达水稻osp5cs1和osp5cs2基因提高烟草脯氨酸的生物合成及其非生物胁迫抗性
DOI: 10.3724/SP.J.1145.2014.03010, PP. 717-722
Keywords: 非生物胁迫,osp5cs,脯氨酸,转基因烟草
Abstract:
为了解水稻osp5cs基因在非生物胁迫中的生物学功能及其生理生化作用，将p5cs基因的两个水稻同源基因osp5cs1和osp5cs2同时构建到植物表达载体phb上，并通过农杆菌介导的遗传转化法转入烟草，经过鉴定转基因植株，成功获得9个独立的转基因阳性植株.选取野生型和其中的3个转基因植株后代，分别测定它们在不同的胁迫条件下幼苗的根长、鲜重以及脯氨酸、过氧化氢和丙二醛的含量.结果显示：在200mmol/lnacl、300mmol/l甘露醇（mannitol）、300μmol/lcuso4胁迫条件下转基因植株的平均根长、平均鲜重、平均脯氨酸、平均过氧化氢含量和平均丙二醛的含量分别为野生型的1.3-2.3、1.9-3.9、1.8-3.2、51%-61%和62%-76%.因此在烟草中过表达osp5cs基因，可以显著增加脯氨酸含量，降低烟草在非生物胁迫条件下的氧化损伤.图6参31
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