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OALib Journal期刊
ISSN: 2333-9721
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温度对厨余垃圾高温厌氧消化及微生物群落的影响
DOI: 10.3724/SP.J.1145.2014.02006, PP. 704-711
Keywords: 厨余垃圾,高温厌氧消化,产甲烷微生物群落,t-rflp分析,克隆文库分析
Abstract:
使用全混流反应器（cstr），研究53℃和60℃条件下厨余垃圾的处理效果，同时利用t-rflp（末端限制性片段长度多态性）以及16srrna基因克隆文库的方法，对微生物群落结构进行跟踪解析.结果表明，在温度为53℃、挥发性总固体（vts）负荷为4gl-1d-1时，处理性能稳定，有机酸积累少，产气率约为900ml/gvts；当温度升高至60℃时，toc和有机酸积累，产气率显著下降.微生物群落分析结果表明，当温度从53℃升高至60℃后，细菌群落从以发酵产酸菌群为主转变为以各类发酵菌和有机酸氧化菌为主，产甲烷菌群落中乙酸营养型产甲烷菌比例下降，而氢营养型产甲烷菌比例升高.运行温度从53℃升高至60℃后厌氧处理能力下降的主要原因是乙酸营养型产甲烷菌比例下降，乙酸的消耗需要通过乙酸氧化菌和氢营养型的产甲烷菌的协同作用，产甲烷速率降低，从而导致处理能力下降.图3表3参50
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