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OALib Journal期刊
ISSN: 2333-9721
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草菇6-磷酸葡萄糖酸脱氢酶基因可变剪接体克隆与表达分析
DOI: 10.3724/SP.J.1145.2014.04022, PP. 584-589
Keywords: 6pgdh,可变剪接,表达分析,基因结构,草菇
Abstract:
6-磷酸葡萄糖酸脱氢酶（6pgdh）是磷酸戊糖途径的限速酶，影响着细胞生命活动所需要的nadph的产生.为揭示草菇菌丝生长的分子基础，通过克隆草菇6-磷酸葡萄糖酸脱氢酶编码基因（6gpdh）的可变剪接体，测序后进行生物信息学分析，利用表达谱和qpcr测定了它们在同核体和异核体菌株的表达量.结果显示，草菇6gpdh序列全长2315bp，含10个内含子，第6个内含子存在内含子保留的可变剪接.在同核体和异核体菌株中，内含子保留的可变剪接体表达量低，且菌株间没显著差异，而内含子被剪接的剪接体表达量高，且异核体菌株高于同核体菌株.两种可变剪接体具有6-磷酸葡萄糖酸脱氢酶保守结构域和相似的三维结构.因此，初步分析两个可变剪切体是同工酶，内含子保留是组成型表达，表达量与菌丝生长快慢无关，内含子剪接是主效剪接方式，菌丝生长快的菌株表达量高，生长慢的菌株表达量低.图6表1参26
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