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OALib Journal期刊
ISSN: 2333-9721
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基于rdnaits和issr标记研究四川松茸遗传多样性
DOI: 10.3724/SP.J.1145.2013.12032, PP. 578-583
Keywords: 松茸,遗传资源,its测序,issr分子标记,聚类分析,地理分化
Abstract:
松茸是一种珍贵的食药用菌，至今无法人工栽培.为更好地开发利用松茸资源，以采自四川小金、雅江、木里等7个县的24株不同生境的野生松茸子实体为材料，采用issr（inter-simplesequencerepeat）分子标记及rdnaits序列分析的方法研究这些菌株的遗传资源及多样性.从15条issr引物中筛选出2条多态性较好的引物对所有菌株进行了扩增，所得条带表明，在松茸中存在一定的遗传多样性；亲缘关系树状图表明，来自木里、会东、小金的大部分松茸样品聚为一个类群，而雅江、盐源、盐边、冕宁的为另一类群，呈现一定的地理分化现象.通过对各菌株rdnaits序列分析表明，松茸菌株间的its序列相似性很高.同时系统进化树表明四川松茸（tricholomamatsutake）与美洲口蘑（t.magnivelare）同源关系较近，与欧洲口蘑（t.caligatum）距离较远，这与报道的形态学分类结果并不一致.本研究表明四川松茸蕴含丰富的遗传信息.图4表4参16
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