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OALib Journal期刊
ISSN: 2333-9721
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gateway技术构建盐和干旱胁迫下水稻根系混合cdna文库及其质量鉴定
DOI: 10.3724/SP.J.1145.2014.00291, PP. 291-294
Keywords: 水稻,抗逆,gateway,cdna文库,盐胁迫,干旱胁迫
Abstract:
为研究水稻非生物胁迫响应信号转导的网络途径，揭示水稻抗逆的分子机制，以nacl胁迫、干旱胁迫处理及正常生长的水稻根系为材料，采用gateway技术构建了水稻根系受盐和干旱诱导的混合cdna文库.cdna文库质量分析表明，未经扩增的原始文库容量为1.5×106cfu,插入片段大小主要为1kb左右，重组率为100%.文库随机挑选克隆测序，结果显示插入序列完整性良好，文库中包含水解酶基因、逆境响应蛋白基因、氧化还原酶基因和富含甘氨酸的rna结合蛋白质基因等.因此，本研究构建的盐和干旱诱导的水稻根系混合cdna文库符合高质量文库的标准，可以用于进一步开展相关基因的克隆及功能研究.
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