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OALib Journal期刊
ISSN: 2333-9721
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多不饱和脂肪酸与粘红酵母低温适应性的关系
DOI: 10.3724/SP.J.1145.2014.00233, PP. 233-237
Keywords: 粘红酵母,低温适应性,多不饱和脂肪酸,膜流动性,δ12-脂肪酸脱氢酶基因
Abstract:
以一株粘红酵母（rhodotorulaglutinis）ym25079为研究对象，分析其低温生长适应性与细胞膜流动性、膜脂脂肪酸含量的变化以及多不饱和脂肪酸合成关键基因――δ12-脂肪酸脱氢酶基因mrna转录水平的关系.结果显示，ym25079在5-30℃温度条件下均能生长，最适生长温度为15℃.在15℃培养条件下，ym25079细胞膜流动性没有明显降低，但是多不饱和脂肪酸含量显著提高，由25℃时29.4%增加到15℃时的55.39%，而且15℃时δ12-脂肪酸脱氢酶基因mrna转录水平提高了5倍.这些研究结果表明，粘红酵母ym25079的低温生长适应性可能是通过提高多不饱和脂肪酸合成相关基因的表达水平，增加膜脂中多不饱和脂肪酸含量，维持低温条件下细胞膜的流动性来形成的.
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