|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
超微铁皮石斛对脾虚便秘小鼠肠道细菌分子多样性的影响
DOI: 10.3724/SP.J.1145.2013.12035, PP. 170-174
Keywords: 脾虚便秘,超微中药,铁皮石斛,肠道细菌多样性,ardra
Abstract:
摘要探讨超微铁皮石斛对脾虚便秘肠道细菌分子多样性的影响，可为其疗效和临床应用提供依据.制备小鼠脾虚便秘模型，设模型组、正常组、铁皮石斛传统汤剂治疗组和超微50%量汤剂治疗组，采集肠道内容物，提取肠道微生物宏基因组，通过细菌通用引物pcr扩增后用ardra分析其多样性.结果表明：正常组和铁皮石斛超微50%量汤剂组的otus（分类操作单元数）、shannon指数和brillouin指数分别相同，依次为6、2.5850和1.5820，模型组和铁皮石斛传统汤剂组的otus、shannon指数和brillouin指数也分别相同，依次为5、2.3219和1.3814，且前两组均大于后两组；铁皮石斛超微50%量汤剂组与正常组群落结构的相似性系数最大，为0.5000，模型组和传统汤剂组较小，为0.1818；聚类分析和主成分分析结果均显示，铁皮石斛超微50%量汤剂组小鼠肠道细菌分子多样性最接近正常组.因此，脾虚便秘造模使小鼠肠道细菌多样性遭到破坏，经铁皮石斛两种汤剂治疗，具有一定的调整作用，超微50%量汤剂效果优于传统汤剂.
| [1] | 11zhengf,fumy,zhaozx,chenmc.communitydiversityofassociatedbacteriaofradopholussimilis[j].plantdispests,2012,3(2):33-37
|
| [2] | 12gaof,yizz,gongj,al-rasheidkhaledas,songwb.molecularphylogenyandspeciesseparationoffivemorphologicallysimilarholosticha-complexciliates(protozoa,ciliophora)usingardrariboprintingandmultigenesequencedata[j].chinjoceanollimnol,2010,28(3):542-548
|
| [3] | 13邹颖,郑学宝,戴世学,叶秋丽,吴丹.小鼠脾虚便秘模型的建立[j].北京中医药,2009,28(1):60-62[zouy,zhengxb,daisx,yeql,wud.establishedmousemodelofspleenconstipation[j].beijingjtradchinmed,2009,28(1):60-
|
| [4] | 14eckburgpb,bikem,bernsteincn,bernsteincn,purdome,dethlefsenl,sargenm,gillsr,nelsonke,relmanda.diversityofthehumanintestinalmicrobialflora[j].science,2005,308:1635-1638.
|
| [5] | 15吴海.超微七味白术散对肠道微生物分子多样性的影响[d].长沙:湖南中医药大学,2012[wuh.effectsofultra-microqiweibaizhusanonmoleculardiversityofintestinalmicrobes[d].changsha:hunanuniversitytcm,
|
| [6] | 16中华人民共和国药典委员会.中华人民共和国药典(一部)[s].北京:中国医药科技出版社,2010:266[pharmacopoeiacommitteeofpeople’srepublicofchina.people’srepublicofchinapharmacopoeia.beijing:chinesemedicalscienceandtechnologypress,2010:
|
| [7] | 17吴海,周赛男,郭纯,谭周进,蔡光先,曾奥,张华玲.一种基于pcr分析多样性的小鼠肠道微生物宏基因组提取方法[j].中国微生态学杂志,2012,24(7):648-651[wuh,zhousn,guoc,tanzj,caigx,zenga,zhanghl.ametagenomednaextractingmethodofintestinalflorainmiceformoleculardiversityanalysisbasedonpcrtechnology[j].chinjmicroecol,2012,24(7):648-
|
| [8] | 23赵兴兵,谢雪姣,吴维佳,张华玲,李丹丹,佘颜,谭周进,蔡光先.超微铁皮石斛对脾虚便秘小鼠肠道乳酸杆菌多样性的影响[j].微生物学报,2014,41(9):1764-1770[zhaoxb,xiexj,wuwj,zhanghl,lidd,shey,tanzj,caigx.effectofultra-micropowderdendrobiumofficinaleonthemoleculardiversityofintestinallactobacillusinmicewithspleen-deficiencyconstipation[j].microbiolchina,2014,41(9):1764-
|
| [9] | 1fernández-ba?aresf.nutritionalcareofthepatientwithconstipation[j].nutrdietaryconsequencesgastronintestinaldis,2006,20(3):575-587
|
| [10] | 2罗迪,庞璐,赵兴兵,蔡光先,吴维佳.浅析《本草纲目》中铁皮石斛的功能主治[j].中国中医杂志,2013,29(3):108-109[luod,pangl,zhaoxb,caigx,wuwj.theindicationsofdendrobiumofficinaleonthe“compendiumofmateriamedica”[j].hunanjtradchin,2013,29(3):108-
|
| [11] | 3王立明,徐建华,陈立钻,孙继军,吴洪海.铁皮枫斗晶对实验性胃阴虚证的药效学研究[j].中成药,2002,10(24):803-805[wanglm,xujh,chenlz,sunjj,wuhh.pharmacodynamicsofdendrobiumcandicumgranulesonexperimentalstomachyindeficiency[j].chintradpatentmed,2002,10(24):803-
|
| [12] | 4daveyme,o’toolega.microbialbiofilms:fromecologytomoleculargenetics[j].microbiolmolbiolrev,2000,64:847-867
|
| [13] | 5hooperlv,wongmh,thelina.molecu-laranalysisofcommensalhost-bacterialrelationshipsintheintestine[j].science,2001,291:881-884
|
| [14] | 6武娜,朱宝利.人体肠道微生物与健康[j].科学(上海),2013,65(2):13-16[wun,zhubl.humangutmicrobesandhealth[j].science(shanghai),2013,65(2):13-
|
| [15] | 7翁幸鐾,糜祖煌.人体肠道微生物群落与疾病[j].公共卫生与临床医学,2010,6(2):82-86[wengxy,mizh.thehumanintestinalmicrofloraanddisease[j].publichealthclinmed,2010,6(2):82-
|
| [16] | 8quigleyemm.theentericmicrobiotainthepathogenesisandmanagementofconstipation[j].bestpracticeresclingastroenterol,2011,25(1):119-126
|
| [17] | 9赵兴兵,吴维佳,李丹丹,李湘玉,张华玲,谭周进,蔡光先.小鼠脾虚便秘造模对肠道微生物及酶活性的影响[j].中国微生态学杂志,2013,25(9):993-996[zhaoxb,wuwj,lidd,lixy,zhanghl,tanzj,caigx.theeffectofmodelingspleen-deficiencyconstipationontheintestinalmicrobiotaandenzymeactivitiesinmice[j].chinjmicroecol,2013,25(9):993-
|
| [18] | 10周健波.利用扩增核糖体基因限制性分析法研究竹鼠盲肠细菌多样性[d].南宁:广西大学,2012[zhoujb.researchrhizomyidaececumbacterialdiversitybyardra[d].nanning:guangxiuniversity,
|
| [19] | 18魏小勇.石斛属植物生物碱研究进展[j].中国药事,2005,19(7):445-447[weixy.reviewontheresearchprogressofalkaloidindendrobiumplants[j].chinpharmaffairs,2005,19(7):445-
|
| [20] | 19李玲,邓晓兰,赵兴兵,曾勇,欧阳冬生.铁皮石斛化学成分及药理作用研究进展[j].肿瘤药学,2011,1(2):90-93[lil,dengxl,zhaoxb,zengy,ouyangds.advancesinstudiesonchemicalconstituentsindendrobiumcandidumandtheirpharmacologicaleffects[j].anti-tumorpharmacy,2011,1(2):90-
|
| [21] | 20李蕾,丁长春,李付惠.两种石斛多糖的抑菌作用研究[j].安徽农业科学,2011,39(10):5753-5754[lil,dingcc,lifh.studyontheantibacterialeffectsoftwodendrobiumpolysaccharides[j].janhuiagricsci,2011,39(10):5753-
|
| [22] | 21张周英,杨成密,蓝忠,黄群,梁林源.石斛多糖的抗菌作用研究[j].中国医药指南,2012,10(33):439-440[zhangzy,yangcm,lanz,huangq,liangly.effectofdendrobiumpolysaccharidesonantimicrobial[j].guidechinamed,2012,10(33):439-
|
| [23] | 22查学强,王军辉,潘利华,罗建平,吕明明.石斛多糖体外抗氧化活性的研究[j].食品科学,2007,10(28):92-93[zhaxq,wangjh,panlh,luojp,lumm.studyonantioxidantactivityofpolysaccharidesfromdendrobiumspecies[j].foodsci,2007,10(28):92-
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
