|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
中国新疆火焰山土壤环境及细菌群落结构
DOI: 10.3724/SP.J.1145.2014.06034, PP. 155-163
Keywords: 新疆,火焰山,肥力评价,土壤酶,土壤微生物,细菌群落结构,dgge
Abstract:
为评价火焰山地区土壤环境现状，并了解环境因子对土壤微生物数量和细菌群落结构的影响，测定了土壤理化性质和4种典型土壤酶活性，结合传统分离培养计数所得微生物数量进行主成分分析（principalcomponentanalysis，pca）和相关性研究，运用变性梯度凝胶电泳（denaturinggradientgelelectrophoresis，dgge）技术结合冗余分析（redundanceanalysis，rda）解析影响细菌群落结构的关键因子.结果表明：（1）火焰山地区土壤肥力状况较差，主要特点为含水率低、养分贫瘠和盐碱化严重，其中盐分含量是限制土壤肥力水平的关键因素，脱氢酶活性可作为快速评价火焰山土壤肥力的重要指标，多酚氧化酶活性最能代表火焰山土壤系统内的肥力变异状况；（2）火焰山土壤微生物数量处于较低水平，细菌为绝对优势菌群（占98.13%），土壤水分是决定微生物数量的关键因子，但盐分含量与微生物数量的相关性不明显；（3）环境因子与土壤细菌群落多样性和结构分布的相关性密切，全氮含量和土壤水分是影响细菌群落多样性的关键因子，各环境因子对不同细菌群落的影响迥异，其共同作用决定了火焰山地区细菌群落结构分布.
| [1] | 35uphoffhu,felskea,fehrw,wagner-dobleri.themicrobialdiversityinpicoplanktonenrichmentcultures:amolecularscreeningofmarineisolates[j].femsmicrobioecol,2001,35:249-258
|
| [2] | 36yeonsh,jeongwj,parkjs.thediversityofculturableorganotrophicbacteriafromlocalsolarsalterns[j].jmicrobiol,2005,43:1-10
|
| [3] | 37吴涛,依艳丽,谢文军,许杰,姚志刚,李小彬,王君.产生物表面活性剂耐盐菌的筛选鉴定及其对石油污染盐渍化土壤的修复作用[j].环境科学学报,2013,33(12):3359-3360[wut,yiyl,xiewj,xuj,yaozg,lixb,wangj.isolationandidentificationofhalotolerantbiosurfactant-productingbacteriaanditsapplicationinbioremediationofpetroleum-contaminatedsalinesoil[j].actascicircumst,2013,33(12):3359-
|
| [4] | 38刘力嘉,马玉龙,谢丽,张新莎.泰乐菌素降解菌的分离鉴定及其系统发育分析[j].应用与环境生物学报,2012,18(6):1038-1042[isolation,identificationandphylogeneticanalysisofatylosin-degradingstrain[j].chinjapplenvironbiol,2012,18(6):1038-
|
| [5] | 39baldrianp,merhautováv,petránkovám,cajthamlt,?najdrj.distributionofmicrobialbiomassandactivityofextracellularenzymesinahardwoodforestsoilreflectsoilmoisturecontent[j].applsoilecol,2010,46(2):177-182
|
| [6] | 3王林霞,王秀云,潘惠云,田长彦.一株新的化能无机营养菌――聚积碳酸镁的细菌[j].干旱区研究,2003,20(1):11-15[wanglx,wangxy,panhx,tiancy.anewcolonyofchemilithotrophicbacterium―abacteriumofgatheringmgco3[j].aridzoneres,2003,20(1):11-
|
| [7] | 4黄山,孟醒,毛虎章,刘赵昆,杨玮,刘方.新疆火焰山土壤中耐高温菌株的分离与鉴定[j].南开大学学报,2011,44(5):109-110[huangs,mengx,maohz,liuzk,yangw,liuf.isolationandidentificationofthermophilicbacteriafromthesoilofxinjiangflamingmountain[j].actascinatunivnankaiensis,2011,44(5):109-
|
| [8] | 5活泼,茆军,石玉瑚.嗜热芽孢杆菌xjt9503高温中性蛋白酶的中式发酵工艺[j].无锡轻工大学学报,2003,22(3):89-92[huop,maoj,shiyh.studyonthepilotfermentationtechnologyofthexjt9503thermostableneutralprotease[j].jwuxiunivlightind,2003,22(3):89-
|
| [9] | 6jánosi,szathmáryl,nádase,bénia,dinyaz,máthée.evaluationofelementalstatusofancienthumanbonesamplesfromnortheasternhungarydatedtothe10thcenturyadbyxrf[j].nuclinstrummethods,2011,269(21):2593-2599
|
| [10] | 7chensk,edwardsca,sublers.theinfluenceoftwoagriculturalbiostimulantsonnitrogentransformations,microbialactivity,andplantgrowthinsoilmicrocosms[j].soilbiolbiochem,2003,35:9-19
|
| [11] | 8kotroczóz,veresz,feketei,krakompergerz,tóthja,lajthak,tóthmérészb.soilenzymeactivityinresponsetolong-termorganicmattermanipulation[j].soilbiolbiochem,2014,70:237-243
|
| [12] | 9lebrunjd,trinsoutrot-gattini,vinceslas-akpam,bailleulc,braulta,mouginc,lavalk.assessingimpactsofcopperonsoilenzymeactivitiesinregardtotheirnaturalspatiotemporalvariationunderlong-termdifferentlanduses[j].soilbiolbiochem,2012,49:150-156
|
| [13] | 10rasooln,reshiza,shahma.effectofbutachlor(g)onsoilenzymeactivity[j].eurjsoilbiol,2014,61:94-100
|
| [14] | 26刘新春,吴成强,杨清香,杨敏.pcr-dgge方法的建立及其在微生物生态群落分析中的应用探讨[c]//张洪勋,庄绪亮.微生物生态学研究进展――第五届微生物生态学术研讨会论文集.北京:气象出版社,2003:32-40
|
| [15] | 1bertoldoc,antranikiang.starch-hydrolysingenzymesfromthermophilicarchaeaandbacteria[j].curropinchembiol,2002,6:151-160
|
| [16] | 2王林霞,王秀云,潘惠云.新疆吐鲁番盆地极端环境微生物资源研究――吐鲁番盆地土壤微生物的生态分布[j].干旱区研究,1991,1:43-46
|
| [17] | 11张焱华,吴敏,何鹏,佘贵连,吴炳孙,韦家少.土壤酶活性与土壤肥力关系的研究进展[j].安徽农业科学,2007,35(34):11139-11142[zhangyh,wum,hep,shegl,wubs,weijs.researchadvanceoftherelationshipbetweensoilenzymeactivityandsoilfertility[j].janhuiagricsci,2007,35(34):11139-
|
| [18] | 12王启兰,王长庭,刘伟,曹广民,龙瑞军.江河源区人工草地植物群落和土壤酶活性变化[j].应用与环境生物学报,2010,16(5):662-666[wangql,wangct,liuw,caogm,longrj.changesinplantcommunitiesandsoilenzymeactivitiesofartificialgrasslandsinheadwaterareasoftheyangtzeandyellowrivers[j].chinjapplenvironbiol,2010,16(5):662-
|
| [19] | 13gil-sotresf,trasar-cepedac,leirosmc,seoanes.differentapproachestoevaluatingsoilqualityusingbiochemicalproperties[j].soilbiolbiochem,2005,37(5):877-887
|
| [20] | 14websterr,oliverma.statisticalmethodsinsoilandlandresourcesurvey[m].oxford:oxforduniversitypress,1990
|
| [21] | 15鲍士旦.土壤农化分析[m].3版.北京:中国农业出版社,2000[baosd.soilandagriculturalchemistryanalysis[m].3rded.beijing:chinaagriculturepress,
|
| [22] | 16李振高,骆永明,腾应.土壤与环境微生物研究法[m].北京:科学出版社,2008
|
| [23] | 17程丽娟,薛泉宏.微生物学实验技[m].西安:世界图书出版公司,2000[chenglj,xueqh.laboratorymanualofmicrobiology[m].xi’an:theworldpublishingcorporation,
|
| [24] | 18yuzt,morrisonm.comparisonsofdifferenthypervariableregionsofrrsgenesforuseinfingerprintingofmicrobialcommunitiesbypcrdenaturinggradientgelelectrophoresis[j].applenvironmicrob,2004,70(8):4800-4806
|
| [25] | 19陈法霖,张凯,郑华,林学强,欧阳志云,屠乃美.pcr-dgge技术解析针叶和阔叶凋落物混合分解对土壤微生物群落结构的影响[j].应用与环境生物学报,2011,17(2):145-150[chenfl,zhangk,zhengh,linxq,ouyangzy,tunm.analyzingtheeffectofmixeddecompositionofconiferandbroadleaflittersonsoilmicrobialcommunitiesbyusingrcr-dgge[j].chinjapplenvironbiol,2011,17(2):145-
|
| [26] | 20唐杰,徐青锐,王立明,丁祥,汤博,吴俐莎,冯�,孙群,杨志荣,张杰.若尔盖高原湿地不同退化阶段的土壤细菌群落多样性[j].微生物学通报,2011,38(5):677-686[tangj,xuqr,wanglm,dingx,tangb,wuls,fengs,sunq,yangzr,zhangj.soilbacterialcommunitydiversityunderdifferentstagesofdegradationinzoigewetland[j].microbiolchina,2011,38(5):677-
|
| [27] | 21李小林,辜运富,张小平,涂仕华,伍仁军.烟草成熟期根际硝化细菌种群的结构及其多样性[j].中国农业科学,2011,44(12):2462-2468[lixl,zhangyf,zhangxp,tush,wurj.nitrobacteriacommunityanddiversityintherhizospheresoilofmaturetocacco[j].sciagricsin,2011,44(12):2462-
|
| [28] | 22bellt,agerd,songj,newmanja,thompsonp,lilleyak,gastcj.lagerislandshousemorebacterialtaxa[j].science,2005,308(5730):1884
|
| [29] | 23lixl,penttinenp,guy,zhangx.diversityofnifhgeneinrhizosphereandnon-rhizospheresoilofobaccoinpanzhihua,china[j].annmicrobiol,2012,62:995-1001
|
| [30] | 24全国土壤普查办公室.中国土壤[m].北京:中国农业出版社,1998
|
| [31] | 25李华,陈英旭,梁新强,田光明,俞巧钢.土壤脲酶活性对稻田田面水氮素转化的影响[j].水土保持学报,2006,20(1):55-58[lix,chenyx,liangxq,tiangm,yuqg.influenceofsoilureaseactivitiesonnitrogenconversioninfloodwaterinpaddyfield[j].jsoilwaterconvers,2006,20(1):55-
|
| [32] | 27罗倩,黄宝灵,唐治喜,来利明,魏伟,郑元润.新疆盐渍土3种植被类型土壤微生物碳源利用[j].应用与环境生物学报,2013,19(1):96-106[luoq,huangbl,tangzx,lailm,weiw,zhengyr.carbonsourceutilizationofmicrobesinsalinesoilofthreevegetationtypesinxinjiang,china[j].chinjapplenvironbiol,2013,19(1):96-
|
| [33] | 28林海明.如何用spss软件一步算出主成分得分值[j].统计与信息论坛,2007,22(5):15-17[linhm.howtousespsssoftwarecalculatethevalueoftheprincipalcomponentscoresinonestep[j].statsinformforum,2007,22(5):15-
|
| [34] | 29李小胜,陈珍珍.如何正确应用spss软件做主成分分析[j].统计研究,2010,27(8):105-108[lixs,chenzz.correctlyusingspsssoftwareforprincipalcomponentsanalysis[j].statres,2010,27(8):105-
|
| [35] | 30yanqy,yuyh,fengws.planktoncommunitycompositioninthethreegorgesreservoirregionrevealedbypcr-dggeanditsrelationshipswithenvironmentalfactors[j].jenvironsci,2008,20:732-738
|
| [36] | 31杨万勤,王开运.土壤酶研究动态与展望[j].应用与环境生物学报,2002,8(5):564-570[yangwq,wangky.advancesonsoilenzymology[j].chinjapplenvironbiol,2002,8(5):564-
|
| [37] | 32曹慧,孙辉,杨浩,孙波,赵其国.土壤酶活性及其对土壤质量的指示研究进展[j].应用与环境生物学报,2003,9(1):105-109[caoh,sunh,yangh,sunb,zhaoqg.areviewsoilenzymeactivityanditsindicationforsoilquality[j].chinjapplenvironbiol,2003,9(1):105-
|
| [38] | 33唐玉姝,慈恩,颜廷梅,魏朝富,杨林章,沈明星.太湖地区长期定位试验稻麦两季土壤酶活性与土壤肥力关系[j].土壤学报,2008,45(5):1000-1006[tangys,cie,yantm,weicf,yanglz,shenmx.relationshipbetweensoilenzymeactivityandsoilfertilityofpaddyfieldsunderwater-ricecroppingsysteminalong-termexperimentintaihulakeregion[j].actapedolsin,2008,45(5):1000-
|
| [39] | 34eilersh,pernthalerj,amannr.successionofpelagicmarinebacteriaduringenrichment:acloselookatcultivation-inducedshifts[j].applenvironmicrobiol,2000,66:4634-4640
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
