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OALib Journal期刊
ISSN: 2333-9721
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环境微生物中芳环加氧酶的获取策略
DOI: 10.3724/SP.J.1145.2012.00880, PP. 880-887
Keywords: 芳环加氧酶获取策略,纯培养技术,宏基因组技术,评价,环境微生物
Abstract:
芳环加氧酶可催化芳香环的羟基化反应，广泛应用于生物修复及化工合成行业.本文在综述芳环加氧酶的分类和应用的基础上，详细探讨基于纯培养技术与宏基因组技术的芳环加氧酶开发策略，并首次对这些策略进行比较和评估.利用传统的纯培养技术已经获得大量的芳环加氧酶，然而这些加氧酶种类单一，重复率高，且新颖性不足.新兴的宏基因组技术赋予了开发未培养微生物资源的新思路，但利用其获取芳环加氧酶的研究刚刚起步，存在着筛选效率低、异源表达困难等难题.本文从基因信息量、筛选通量与效率、时间与成本以及目的基因下游表达可行性等方面对这两种方法进行综合评价，并提出宏基因组技术筛选不同类型芳环加氧酶的适用方法.纯培养技术与宏基因组技术的有机结合，与蓬勃发展的各种组学技术一起，将有助于推动芳环加氧酶的理论研究及生物催化产业的快速发展.图3表2参47
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