|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
水稻叶片蛋白质组双向电泳实验条件的改进
DOI: 10.3724/SP.J.1145.2012.00819, PP. 819-823
Keywords: 水稻,双向电泳,蛋白质组学,5%冰乙酸,考马斯亮蓝r-250
Abstract:
为获得高分辨率、高灵敏度的2-de图谱，以3叶1心期的水稻幼苗叶片为研究材料，对蛋白样本的制备、上样量及考马斯亮蓝r-250染色等关键步骤进行探讨，优化实验条件.结果表明，脱色后凝胶经5%冰乙酸浸泡后得到的2-de图谱蛋白质点明显增多，图谱背景清晰.同时，不同公司考染试剂cbbr-250染色灵敏度存在较大的差别，应予以尝试.同其它方法相比，本方法简便、稳定，灵敏度高，可操作性强，对水稻蛋白质组学研究具有一定的指导意义.图4参15
| [1] | 4rakwalr,agrawalgk.riceproteomics:currentstatusandfutureperspectives.electrophoresis,2003,24(19~20):3378~3389
|
| [2] | 5torabis,wissuwam,heidarim,naghavimr,gilanyk,hajirezaeimr,omidim,yazdi-samadib,ismailam,salekdehgh.acomparativeproteomeapproachtodecipherthemechanismofriceadaptationtophosphorousdeficiency.proteomics,2009,9(1):159~170
|
| [3] | 6yangp,liangy,shens,kuangt.proteomeanalysisofriceuppermostinternodesatthemilkystage.proteomics,2006,6(11):3330~3338
|
| [4] | 7o’farrellph.highresolutiontwo-dimensionalelectrophoresisofproteins.jbiolchem,1975,250(10):4007~4021
|
| [5] | 8gallardok,jobc,grootspc,puypem,demolh,vandekerckhovej,jobd.proteomicsofarabidopsisseedgermination:acomparativestudyofwild-typeandgibberellindeficientseeds.plantphysiol,2002,129(5):823~837
|
| [6] | 9dingcq(丁承强),mad(马丹),wangsh(王绍华),dingyf(丁艳锋).optimizationprocessandmethodof2-delectrophoresisforriceproteomics.chinbullbot(植物学报),2011,46(1):67~73
|
| [7] | 10wangyq(王玉琪),pengxx(彭新湘).atwo-dimensionalelectrophoresisprotocolsuitableforproteomicstudyofriceleaves.jplantphysiol&molbiol(植物生理与分子生物学学报),2006,32(2):252~256
|
| [8] | 11liangsj(梁书剑),zhangm(张萌),wangw(王巍),guangsh(关双红),sunyq(孙野青).proteinsfromriceleavesextractedwithmg/np240bufferaredifferentfromthosewithtcabuffer.jbiol(生物学杂志),2009,26(3):68~71
|
| [9] | 12yucl(余初浪),yansp(严顺平),sunwp(孙卫宁),yangl(杨玲).high-resolutiontwo-dimensionalelectrophoresisfortotalproteinsinriceroots,leavesandsuspensioncells.chinjricesci(中国水稻科学),2006,20(5):549~552
|
| [10] | 13bradfordmm.arapidandsensitivemethodforthequantizationofmicrogramquantitiesofproteinutilizingtheprincipleofprotein-dyebinding.analbiochem,1976,72(1~2):248~254
|
| [11] | 14tsugitaa,kamom.2-delectrophoresisofplantproteins.methodsmolbiol,1999,112:95~97
|
| [12] | 15pasqualic,fialkali,huberla.preparativetwodimensionalgelelectrophoresisofmembraneproteins.electrophoresis,1997,18(14):2573~2581
|
| [13] | 1chittetibr,pengz.proteomeandphosphoproteomedifferentialexpressionundersalinitystressinrice(oryzasativa)roots.jproteomeres,2007,6(5):1718~1727
|
| [14] | 2fanwh,cuiwt,lixf,chensy,liugs,shensh.proteomicsanalysisofriceseedlingresponsestoovinesaliva.jplantphysiol,2011,168(5):500~509
|
| [15] | 3nwugocc,huertaaj.theeffectofsiliconontheleafproteomeofrice(oryzasatival.)plantsundercadmium-stress.jproteomeres,2011,10(2):518~528
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
