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OALib Journal期刊
ISSN: 2333-9721
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产甘油假丝酵母补料发酵中的甘油合成衰减
DOI: 10.3724/SP.J.1145.2012.00791, PP. 791-796
Keywords: 产甘油假丝酵母,补料发酵,甘油合成衰减,代谢流,关键酶
Abstract:
为揭示产甘油假丝酵母candidaglycerinogenes补料发酵后甘油合成衰减机理，以指导甘油合成及菌株改造，对补料发酵后甘油的生成情况、葡萄糖的消耗情况、细胞内的代谢流量变化以及代谢途径关键酶活力变化进行了研究.结果表明，补料发酵后葡萄糖的代谢流量分布发生变化，流向hmp途径和甘油合成途径的流量分别降低48%和33%，更多的碳流通过emp途径形成副产物；胞浆3-磷酸甘油脱氢酶（ctgpd）和6-磷酸葡萄糖脱氢酶（g6pdh）的活力下降，而丙酮酸激酶（pyk）活力上升.补料发酵后甘油合成关键酶ctgpd酶活力低下，流向甘油合成途径的碳流减小，还原力供给受影响，这些因素共同作用引起了补料发酵后甘油合成的衰减.图4表2参22
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