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浓香型白酒窖泥微生物群落plfa指纹图谱方法

DOI: 10.3724/SP.J.1145.2012.00831, PP. 831-837

Keywords: 磷脂脂肪酸,窖泥,微生物群落,指纹图谱,主成分分析,浓香型白酒

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Abstract:

针对白酒发酵过程中窖泥微生物群落的特殊性，以不同类型泥土和常见酿酒微生物为研究对象，构建了研究浓香型白酒窖泥微生物群落的磷脂脂肪酸（plfa）指纹图谱法.结果表明：不同类型样品plfa指纹图谱的显著差异（p<0.05）体现为样品plfa的组成及含量受其生理及环境条件的影响，发生了显著的变化（花园和路旁泥土的真菌与细菌特征plfa的比值分别为0.30和0.66，均小于1，而窖泥为1.17）；5种常见微生物纯培养物加入窖泥后使得与其类型相对应的特征plfa含量变化显著（添加sicc1.13的窖泥中a14:0增加了1.44倍，cicc20633使a16:0增加了9.92倍，sicc3398使18:2ω6,9增加了82.58倍，sicc31482使18:2ω6,9增加了90.33倍，添加dh5α的窖泥中检出了cy17:0）（p<0.05）；应用主成分分析（pca）揭示了窖泥中加入不同微生物菌株后其特征plfa的组成及含量的变化规律.研究佐证了plfa指纹图谱法是一种表征酿酒过程微生物群落指纹图谱快捷的生化免培养法.图4表1参31

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