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OALib Journal期刊
ISSN: 2333-9721
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喷雾冷凝法制备高性能乳酸菌微胶囊
DOI: 10.3724/SP.J.1145.2012.00824, PP. 824-830
Keywords: 微胶囊,乳酸菌,喷雾冷凝法,变性淀粉,胃肠道存活率
Abstract:
活菌制剂进入动物消化道后由于经受胃酸、胆盐以及消化酶等的作用而造成活性丧失及肠道定殖困难是目前乳杆菌制剂应用的瓶颈问题.本研究通过喷雾冷凝法制备干酪乳杆菌微胶囊以增强乳杆菌在人体的益生功能.选用不同浓度的海藻酸钠溶液与氯化钙溶液作为壁材，考察喷雾冷凝法制备乳杆菌微胶囊的包埋率、胃肠道释放等情况.通过比较微胶囊的包埋率和粒径分布的效果后，选择海藻酸钠浓度为2%、氯化钙浓度为3%的芯壁材（包埋率最高达95.80%）制备干酪乳杆菌微胶囊.初始活菌数为109cfu/ml的乳酸菌微胶囊经模拟人体胃液处理3h后，仅有24.17%的活菌溢出，证明微胶囊处理后的乳杆菌能够抵抗较低ph值及胃蛋白酶的作用.之后将微胶囊置入模拟人体肠液中，60min后微胶囊释放率达到84.22%，表明上述方法制备的乳杆菌微胶囊能够在肠道中释放定殖并发挥其益生作用.进一步研究表明，添加变性淀粉作为益生元，能够增强乳酸菌微胶囊的耐酸性能，并且在肠液中释放后活菌出现继续增殖的现象.上述研究结果为选择高效保护剂应用喷雾冷凝法制备乳酸菌微胶囊奠定了研究基础.图4表2参34
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