|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
青海湖嗜盐菌的分离与优势菌株qhl5的特性
DOI: 10.3724/SP.J.1145.2012.00797, PP. 797-803
Keywords: 青海湖,中度嗜盐菌,分子鉴定,樊氏盐单胞菌,tlc法,四氢嘧啶
Abstract:
采用高盐选择性培养基从20份青海湖水样中筛选分离获得35株青海湖嗜盐微生物.耐盐梯度实验分析表明：水体中以中度嗜盐菌为主，约占65.7%；弱嗜盐菌次之，约占25.7%；非嗜盐菌与耐盐菌相对较少，约占8.5%.其中优势菌qhl5的生长盐度耐受范围为0.04~3.0mol/l，最适生长范围为0.3~0.9mol/l，隶属于中度嗜盐菌.菌体形态和生长特性分析表明：qhl5菌落大小为4~6mm，圆形或椭圆形，边缘整齐厚实，乳白色，中间微隆起，不透明，革兰氏染色阴性.显微形态呈杆状，最适生长ph为8.5~9.5，最适生长温度为35℃.生理生化、16srrna的全序列blast和系统发育分析表明：qhl5菌株分类定位于γ变形菌纲海洋螺菌目盐单胞菌属樊氏盐单胞菌（halomonasventosae）.通过80%乙醇法抽提胞内相溶物质，采用tlc法检测出该菌积聚四氢嘧啶.图6表1参28
| [1] | 1caowj(曹卫军),shenp(沈萍),licy(李朝阳).extremmophiles.wuhan:wuhanuniversitypress(武汉:武汉大学出版社),2004.1~19
|
| [2] | 7fengem(冯二梅),suhy(宿红艳),wangl(王磊).isolation,identificationandphylogeneticanalysisofmoderatelyhalophilicbacteriumisolatedfromyantaiseaarea.marscibull(海洋通报),2010,29(1):52~58
|
| [3] | 8zhangxm(张晓梅),douwf(窦文芳),xuhy(许泓瑜),xuzh(许正宏).identificationofalipaseproducinghalophilicstrainanditshalophilicmechanism.chinjapplenvironbiol(应用与环境生物学报),2010,16(1):100~103
|
| [4] | 12liux(刘旭光),zhangj(张杰).molecularbiologysoftwareapplication.beijing:beijinguniversitymedicalpress(北京:北京医学出版社),2007
|
| [5] | 13anneuk,erhardb.osmoticallyregulatedsynthesisofthecompatiblesoluteectoineinbacilluspasteuriiandrelatedbacillusspp.applenvironmicrobiol,2002,68(2):772~7831
|
| [6] | 15jiangwy(姜蔚宇),chenzl(陈志亮),fuyn(傅英楠),chenrz(陈荣忠).isolationandcharacterizationofanectoine-producingmoderatehalophilicbacteriumta-4.joceanogrtaiwanstrait(台湾海峡),2008,27(2):168~173
|
| [7] | 16wangh(汪辉),zhoucl(周长林).isolationandidentificationofastrainofhalomonasandresearchonactivitiesofitsproduct.progpharmsci(药学进展),2005,29(6):266~270
|
| [8] | 17xiongy(熊焰),wup(吴鹏),youff(尤芳芳),ruank(阮琨),caoy(曹毅),qiaodr(乔代蓉),bailh(白林含).isolationandcharacterizationofamoderatelyhalophilicbacteriumhalomonassp.ny-011.jsichununiv(四川大学学报),2008,45(5):1239~1244
|
| [9] | 18josemc,emiliaq,inmaculadal.halomonasventosaesp.nov,amoderatelyhalophilic,denitrifying,exopolysaccharide-producingbacterium.intjsystevolmicrobiol,2004,54:733~737
|
| [10] | 19daochenz,lilin,chenxiangw.solationandcharacterisationofmoderatelyhalophilicbacteriumhalomonasventosaedl7synthesizingectoineascompatiblesolute.annmicrobiol,2007,57(3):401~406
|
| [11] | 20carmenmg,fernandomc.halomonascerinasp.nov.,amoderatelyhalophilic,denitrifying,exopolysaccharide-producingbacterium.intjsystevolmicr,2008,58:803~809
|
| [12] | 21carmenmg,victoriab.halomonasnitroreducenssp.nov.,anovelnitrateandnitrite-reducingspecies.intjsystevolmicr,2008,58:872~876
|
| [13] | 22yananw,huac,changqiaoc.halomonasshengliensissp.nov.,amoderatelyhalophilic,denitrifying,crudeoilutilizingbacterium.intjsystevolmicr,2007,57:1222~1226
|
| [14] | 23hongbl,leipz,sanfc.halomonaskorlensissp.nov.,amoderatelyhalophilic,denitrifyingbacteriumisolatedfromsalineandalkalinesoil.intjsystevolmicr,2008,58:2582~2588
|
| [15] | 24zhudc(朱道辰),liuxr(刘杏荣).compatiblesolutesectioneanditsderivatehydroxyectoine.chinbiotechnol(中国生物工程杂志),2011,31(2):95~101
|
| [16] | 25zhangk(张琨),lanjh(蓝江湖),shenzx(沈振兴),xuh(徐海).thechemicalcompositionandqualityevaluationofsurfacewaterinqinghailakeareas.jearthenviron(地球环境学报),2010,1(3):162~168
|
| [17] | 26tuojc(妥进才),chenj(陈骏),yaosp(姚素平),jijf(季峻峰),liyl(李一良),zhangcl(张传伦).qinghailake:anaturallaboratoryforgeomicrobiologicalresearch.geoljchinuniv(高校地质学报),2005,11(2):187~193
|
| [18] | 2zhangel(张恩楼),shenj(沈吉),wangsm(王苏民),yiny(尹宇),zhuyx(朱育新),xiawl(夏威岚).quantitativereconstructionofqinghailakesalinityduringthepast0.9ka.chinscibull(科学通报),2004,49(7):697~701
|
| [19] | 3zhuwj(朱文杰),wangj(汪杰),chenxy(陈晓耘),zhaxicr(扎西次仁),yangy(杨韵),songy(宋瑛).anewspeciesofluminousbacteriavibrioqinghaiensissp.nov.oceanollimnolsin(海洋与湖沼),1994,25(3):273~280
|
| [20] | 4miq(米琴),any(安燕),haow(郝微),hey(何艳),zhuwj(朱文杰).isolationandidentificationofluminousbacteriafromtheqinghailakeandobservationbyelectronicmicroscope.jeastchinanormaluniv(华东师范大学学报),2008,4:58~65
|
| [21] | 5jiaoyc(焦迎春).isolationandpurificationofpsychrophilefromqinghailakeandbiochemicalcharacteristic.jqinghaiuniv(青海大学学报),2008,26(3):11~14
|
| [22] | 6houmf(侯梅锋),hesl(何士龙),lid(李栋),zhangj(张洁),zhaoy(赵云).bacterialdiversityinlianyungangmarinesedimentandqinghailakesediment.environsci(环境科学).2011,32(9):2681~2688
|
| [23] | 9dongxz(东秀珠),caimy(蔡妙英).systemidentificationmanualofcommonlyfoundbacterium.beijing:sciencepress(北京:科学出版社),2001
|
| [24] | 10buchananre,gibbonsne.bergey’smanualofsystemaicbacteriology.8thed.beijing:sciencepress(北京:科学出版社),1984
|
| [25] | 11xiey(谢莹),linlb(林连兵),jixl(季秀玲),weiyl(魏云林).isolationand16srrnagenesequenceanalysisofathermotoleranthalophilicbacterium.microbiolbull(微生物学通报),2008,35(2):166~170
|
| [26] | 14fuyn(傅英楠),chenzl(陈志亮),jiangwy(姜蔚宇),chenrz(陈荣忠).isolationandcharacteristicofamoderatelyhalophilicbacteriumnjs2accumulatedectoinefromantarcticadeepseasediment.jxiamenuniv(厦门大学学报),2008,47(3):4~6
|
| [27] | 27kokih,garoa,alantb.extremophileshandbook.springer,2011,doi10.1007/978
|
| [28] | 28taots(陶天申),yangrf(杨瑞馥),dongxz(东秀珠).systematicsofprokaryotes.beijing:chemicalindustrypress(北京:化学工业出版社),2007
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
