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OALib Journal期刊
ISSN: 2333-9721
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均匀设计法优化高效杀藻菌microbulbifersp.bs03培养基及发酵条件
DOI: 10.3724/SP.J.1145.2012.00761, PP. 761-767
Keywords: 杀藻细菌,单因素试验,均匀设计,塔玛亚历山大藻,发酵条件
Abstract:
采用均匀设计法设计和二次多项式逐步回归分析，对一株高效杀塔玛亚历山大藻微泡菌bs03（microbulbifersp.）产杀藻活性物质的发酵培养条件进行优化.通过单因素实验筛选出碳源、氮源、ph、培养时间和接种量为显著影响因子，并对5个显著影响因子采用u15（155）水平对培养基进行优化.结果表明bs03最适发酵培养条件为：蔗糖8g/l，蛋白胨10.50g/l，初始ph值7.5，培养时间32h，接种量3.00%.验证试验结果显示，在此条件下该菌发酵液的干重为4.725g/l，较优化前增加了31.35%，ld50为0.768%，较优化前降低了25.14%.研究结果为杀藻活性物质以及杀藻机理的研究奠定了理论基础.图7表5参20
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