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OALib Journal期刊
ISSN: 2333-9721
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一株嗜碱产甲烷八叠球菌的分离鉴定与系统发育分析
DOI: 10.3724/SP.J.1145.2012.00262, PP. 262-266
Keywords: 嗜碱产甲烷菌,菌株分离鉴定,厌氧发酵,系统发育分析,16srdna
Abstract:
在15℃条件下用产甲烷菌培养基对采自四川省红原县的牦牛粪进行富集培养，采用hungate厌氧操作技术从富集培养物中分离得到一株在8~45℃范围生长、最适生长ph为8.5的嗜碱产甲烷菌t13.该菌株革兰氏染色阳性，细胞聚集体，在液体培养基中为肉眼可见的颗粒状物，在固体培养基上菌落为淡黄色桑葚状；可利用甲醇、乙酸盐和甲胺作为唯一碳源生长；对氯霉素和庆大霉素敏感；生长ph范围为6.5~9.5；最适nacl浓度为0~0.15moll-1；最适生长温度为30℃.形态和生理生化特征以及16srdna序列分析表明菌株t13为梅氏产甲烷八叠球菌（methanosarcinamazei）.由于该菌最适生长ph为8.5，所以初步认为菌株t13是一株梅氏产甲烷八叠球菌的新菌株.图5表1参18
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