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悬沙胁迫下三疣梭子蟹仔蟹的rna/dna比率和dna损伤

DOI: 10.3724/SP.J.1145.2012.00251, PP. 251-255

Keywords: 悬沙,仔蟹,成活率,rna/dna比率,dna损伤

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Abstract:

为探讨悬沙对仔蟹的影响,以三疣梭子蟹(portunustrituberculatus)仔蟹为受试生物,研究不同悬沙浓度(0、50、100、500、1000和5000mgl-1)下i期仔蟹的成活率、rna/dna比率和dna损伤情况.结果表明,低浓度悬沙(≤500mgl-1)对仔蟹无明显影响,高浓度悬沙(≥1000mgl-1)对仔蟹的成活率几乎无影响,但会延迟仔蟹蜕壳变态的时间,降低rna/dna比率,导致彗星细胞的拖尾率、尾长和尾矩显著上升.可见,rna/dna比率和dna损伤指标能够有效评估悬沙对仔蟹的影响.图5参27

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