|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
番茄arf4基因果实特异rnai载体的构建及遗传转化
DOI: 10.3724/SP.J.1145.2012.00206, PP. 206-211
Keywords: arf4,rna干涉,果实特异表达,遗传转化,转基因番茄,果实品质改良
Abstract:
构建arf4基因果实特异表达的rna干涉载体，对转基因番茄果实进行初步分析，可为采用基因工程方法改良番茄果实品质做出新尝试.利用rt-pcr技术从番茄果实cdna中扩增slarf4基因全长，将番茄arf4基因正反向重复序列片段导入到植物表达载体pbi121上，启动子是番茄果实特异表达的tfm7.将构建的arf4基因果实特异rna干涉载体pbi121-tfm7-a4ri通过根癌农杆菌介导转入到野生型番茄中，进而对转化获得的植株进行了阳性鉴定.分别以转基因番茄和野生型番茄为材料，分析arf4在果实中的表达水平，测定绿熟期果实叶绿素含量、果实的单果重量和果皮厚度.酶切证实pbi121-tfm7-a4ri果实特异表达载体构建成功，而且，pcr检测也得到阳性转基因株.半定量rt-pcr分析显示，转基因番茄果实中arf4的表达量明显低于野生型果实.转基因番茄果实的叶绿素含量、单果重量和果皮厚度都比野生型有提高.因此，arf4果实特异表达的rnai方法能够改良番茄果实品质.图7表2参21
| [1] | 4pekkeri,alvarezjp,eshedy.auxinresponsefactorsmediatearabidopsisorganasymmetryviamodulationofkanadiactivity.plantcell,2005,17:2899~2910
|
| [2] | 5sessionsra,zambryskipc.arabidopsisgynoeciumstructureinthewildandinettinmutants.development,1995,121:1519~1532
|
| [3] | 7liuy,roofs,yez,barryc,vantuinena,vrebalovj,owlerc,giovannonij.manipulationoflightsignaltransductionasameansofmodifyingfruitnutritionalqualityintomato.procnatlacadsciusa,2004,101:9897~9902
|
| [4] | 8davulurigr,vantuinena,fraserpd,manfredoniaa,newmanr,burgessd,brummellda,kingsr,palysj,uhligj,bramleypm,penningshm,bowlerc.fruit-specificrnai-mediatedsuppressionofdet1enhancescarotenoidandflavonoidcontentintomatoes.natbiotechnol,2005,23:890~895
|
| [5] | 9longm,millardj,kimuray,donovang,reesj,fraserpd,brameleypm,bolwellgp.metaboliteprofilingofcarotenoidandphenolicpathwaysinmutantandtransgeniclinesoftomato:identificationofahighantioxidantfruitline.phytochemistry,2006,67:1750~1757
|
| [6] | 16allene,xiezx,gustafsonam,carringtonjc.microrna-directedphasingduringtrans-actingsirnabiogenesisinplants.cell,2005,121:207~221
|
| [7] | 20wangsh,liujk,fengyy,niuxl,giovannonij,liuys.alteredplastidlevelsandpotentialforimprovedfruitnutrientcontentbydownregulationofthetomatoddb1-interactingproteincul4.plantj,2008,55:89~103
|
| [8] | 1guilfoyletj,hageng.auxinresponsefactors.curropinplantbiol,2007,10:453~460
|
| [9] | 2liscume,reedjw.geneticsofaux/iaaandarfactioninplantgrowthanddevelopment.plantmolbiol,2002,49:387~400
|
| [10] | 3yoonek,yangjh,limj,kimsh,kimsk,leews.auxinregulationofthemicrorna390-dependenttransactingsmallinterferingrnapathwayinarabidopsislateralrootdevelopment.nucleicacidsres,2010,38:1382~1391
|
| [11] | 6jonesb,frassep,olmose,zegzoutih,lizg,latche´a,pechjc,bouzayenm.down-regulationofdr12,anauxin-response-factorhomolog,inthetomatoresultsinapleiotropicphenotypeincludingdarkgreenandblotchyripeningfruit.plantj,2002,32:603~613
|
| [12] | 10liujk(刘继恺),gaoyf(高永峰),niuxl(牛向丽),liuys(刘永胜).constructionandtransformationofco-rnaivectoroftomatohp1andhp2genes.chinjapplenvironbiolm(应用与环境生物学报),2009,15:591~595
|
| [13] | 11yint(尹涛),zhangsl(张上隆),liujm(刘敬梅),chendm(陈大明).advancesinfruit-specificpromoteranditsapplications.jagricbiotechnol(农业生物技术学报),2009,17:355~360
|
| [14] | 12chenyh(陈银华),lihx(李汉霞),yezb(叶志彪).effectsofintroductionofdifferentt-dnastructuresofacogenetotomatogenomeonethyleneproductionrate.actahortsin(园艺学报),2007,34:644~648
|
| [15] | 13wangh,jonesb,lizg,latchea,pechjc,bouzayenm.thetomatoaux/iaatranscriptionfactoriaa9isinvolvedinfruitdevelopmentandleafmorphogenesis.plantcell,2005,17:2676~2692
|
| [16] | 14pengys(彭运生),liue(刘恩).studiesofmethodonextractchlorophyllaandb.actaagricunivpekinensis(北京农业大学学报),1992,18:247~250
|
| [17] | 15williamsl,carlescc,osmontks,fletcherjc.adatabaseanalysismethodidentifiesanendogenoustrans-actingshort-interferingrnathattargetsthearabidopsisarf2,arf3,andarf4genes.procnatlacadsciusa,2005,102:9703~9708
|
| [18] | 17mengfj(孟凡娟),xuxy(许向阳),lijf(李景富).studyoncorrelationofthetomatofruit’spigmentcontentandsurfacecolour.jnortheastagricuniv(东北农业大学学报),2006,37:459~462.
|
| [19] | 18gillaspyg,ben-davidh,gruissemw.fruits:adevelopmentalperspective.plantcell,1993,5:1439~1451
|
| [20] | 19guillonf,philippes,bouchetb,devauxmf,frassep,jonesb,bouzayenm,lahayem.down-regulationofanauxinresponsefactorinthetomatoinducesmodificationoffinepectinstructureandtissuearchitecture.jexpbot,2008,59:273~288
|
| [21] | 21himberc,dunoyerp,moissiardg,ritzenthalerc,voinneto.transitivity-dependentand�independentcell-to-cellmovementofrnasilencing.emboj,2003,22:4523~4533
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
