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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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苏云金芽胞杆菌ybt-1520中sigl及其增强子结合蛋白的结构与功能分析
DOI: 10.3724/SP.J.1145.2012.00315, PP. 315-319
Keywords: 苏云金芽胞杆菌,增强子结合蛋白,sigl,代谢途径,功能分析,生物信息学,表达调控
Abstract:
为揭示sigl及其增强子结合蛋白（ebps）在苏云金芽胞杆菌（bt）中的调控功能，在全基因组测序的基础上，采用生物信息学方法，对ybt-1520菌株的sigl及其ebps的结构和功能进行深入分析.结果表明，ybt-1520基因组中存在1个sigl和6个ebps，而且ebps在结构上具有丰富的多样性，包含了ebps的所有可能的结构域组织类型.sigl所调控的基因涉及11个假定的cog代谢途径，其中包括能量代谢、氨基酸代谢、翻译与细胞周期等.根据ebps在基因组的位置推测，ybt-1520的ebps参与γ-氨基丁酸代谢途径、精氨酸代谢途径、支链脂肪酸降解途径、多糖分解代谢等代谢途径的调控.本研究将为揭示bt杀虫晶体蛋白大量表达的调控机制提供新的思路.图2表1参23
| [1] | 7wangh,gunsalusrp.coordinateregulationoftheescherichiacoliformatedehydrogenasefdnghiandfdhfgenesinresponsetonitrate,nitrite,andformate:rolesfornarlandnarp.jbacteriol,2003,185(17):5076~5085
|
| [2] | 8selfwt,hasonaa,shanmugamkt.n-terminaltruncationsinthefhlaproteinresultinformate-andmoea-independentexpressionofthehyc(formatehydrogenlyase)operonofescherichiacoli.microbiology,2001,147(11):3093~3014
|
| [3] | 9leesk,keaslingjd.effectofglucoseorglycerolasthesolecarbonsourceongeneexpressionfromthesalmonellaprpbcdepromoterinescherichiacoli.biotechnolprog,2006,22(6):1547~1551
|
| [4] | 10darwinaj.thephage-shock-proteinresponse.molmicrobiol,2005,57(3):621~628
|
| [5] | 11studholmedj,dixonr.insilicoanalysisofthesigma54-dependentenhancer-bindingproteinsinpirellulaspeciesstrain1.femsmicrobiollett,2004,230(2):215~225
|
| [6] | 12leonhartsbergers,hubera,lottspeichf,bocka.thehydh/ggenesfromescherichiacolicodeforazincandleadresponsivetwo-componentregulatorysystem.jmolbiol,2001,307(1):93~105
|
| [7] | 13mattamk,lioliouee,panaqiotidisch,kyriakidisda,anagiotidisca.interactionsoftheantizymeatocwithregulatoryelementsoftheescherichiacoliatodaeboperon.jbacteriol,2007,189(17):6324~6332
|
| [8] | 14debarbouillem,martin-verstraetei,kliera,rapoportg.thetranscriptionalregulatorlevrofbacillussubtilishasdomainshomologoustobothσ54andphosphotransferasesystem-dependentregulators.procnatlacadsciusa,1991,88(6):2212~2216
|
| [9] | 15alino,bignonj,rapoportg,debarbouillem.regulationoftheacetoincatabolicpathwayiscontrolledbysigmalinbacillussubtilis.jbacteriol,2001,183(8):2497~2504
|
| [10] | 16debarbouillem,gardanr,arnaudm,rapoporg.roleofbkdr,atranscriptionalactivatorofthesigl-dependentisoleucineandvalinedegradationpathwayinbacillussubtilis.jbacteriol,1999,181(7):2059~2066
|
| [11] | 17alino,jeussetj,larquete,lecame,belitskyb,sonensheinal,msadekt,debarbouillem.specificityoftheinteractionofrocrwiththerocg�rocaintergenicregioninbacillussubtilis.microbiology,2003,149(3):739~750
|
| [12] | 18finnrd,mistryj,tatej,coggillp,hegera,pollingtonje,gavinol,gunasekaranp,cericg,forslundk,holml,sonnhammerel,eddysr,batemana.thepfamproteinfamiliesdatabase.nucleicacidsres,2010,38:d211~222
|
| [13] | 1lonettoma,gribskovm,grossca.theσ70family:sequenceconservationandevolutionaryrelationships.jbacteriol,1992,174(12):3843~3849
|
| [14] | 2lonettoma,brownkl,ruddke,buttnermj.analysisofthestreptomycescoelicolorsigegenerevealstheexistenceofasubfamilyofeubacterialrnapolymeraseσfactorsinvolvedintheregulationofextracytoplasmicfunctions.procnatlacadsciusa,1994,91(16):7573~7577
|
| [15] | 3pengq(彭琦).studyonthefunctionofsiglfrombacillusthuringiensis:[masterdegreedissertation].beijing,china:chineseacademyofagriculturalsciences(中国农业科学院),2008
|
| [16] | 4caldaram,minhpn,bostoens,massantj,charlierd.argr-dependentrepressionofarginineandhistidinetransportgenesinescherichiacolik-12.jmolbiol,2007,373(2):251~267
|
| [17] | 5kiupakisak,reitzerl.argr-independentinductionandargr-dependentsuperinductionoftheastcadbeoperoninescherichiacoli.jbacteriol,2002,184(11):2940~2950
|
| [18] | 6samsonovann,smirnovsv,altmanib,ptitsynlr.molecularcloningcharacterizationofescherichiacolik12ygjggene.bmcmicrobiol,2003,3(1):2
|
| [19] | 19barriosh,valderramab,morette.compilationandanalysisofsigma(54)-dependentpromotersequences.nucleicacidsres,1999,27(22):4305~4313
|
| [20] | 20laurenbeckl,smithtg,hoovertr.look,nohands!unconventionaltranscriptionalactivatorsinbacteria.trendsmicrobiol,2007,15(12):530~537
|
| [21] | 21schumacherj,jolyn,rappasm,zhangxd,buckm.structuresandorganisationofaaa+enhancerbindingproteinsintranscriptionalactivation.jstructbiol,2006,156(1):190~199
|
| [22] | 22davidj.studholme1,dixonr.domainarchitecturesofσ54-dependenttranscriptionalactivators.jbacteriol,2003,185(6):1757~1767
|
| [23] | 23zhul,pengq,songfp,jiangyn,suncp,zhangj,huangdf.structureandregulationofthegabgenecluster,involvedintheγ-aminobutyricacidshunt,arecontrolledbyaσ54factorinbacillusthuringiensis.jbacteriol,2010,192(1):346~355
|
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