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OALib Journal期刊
ISSN: 2333-9721
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孔雀石绿和结晶紫在荧光检测中的比较
DOI: 10.3724/SP.J.1145.2013.00365, PP. 365-369
Keywords: 孔雀石绿,结晶紫,g-四聚体,链置换等温扩增,实时荧光检测
Abstract:
为优选出三苯甲烷染料中的一种染料与g-四聚体结合开发为荧光定量报告体系，对孔雀石绿（malachitegreen，mg）和结晶紫（crystalviolet，cv）与g-四聚体结合后的灵敏度和重现性进行了比较研究.首先，比较了4种不同dna（hum21，short-hum21，double-strands，single-strand）分别与孔雀石绿和结晶紫结合后的荧光光谱，结果显示，两种染料均可区分以上4种dna，但孔雀石绿的灵敏度更高.然后，将孔雀石绿和结晶紫应用到实时荧光检测中，进一步比较验证两种染料灵敏度，并考察两种染料的重现性，结果显示，孔雀石绿的重现性和灵敏度均优于结晶紫.由此，建立了孔雀石绿与g-四聚体结合的荧光定量报告体系.图4表1参21
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