|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
体外筛选剪切dna的dna酶
DOI: 10.3724/SP.J.1145.2013.00360, PP. 360-364
Keywords: 体外筛选,dna酶,催化活性,剪切dna
Abstract:
为得到能够剪切单链dna序列的人工dna酶，通过体外筛选的方法，得到了一类dna酶f-9.f-9的结构由两侧与底物结合区域和中间的酶活性中心组成；f-9在一定的cu2+、mn2+离子浓度下发挥催化作用，可以与底物结合形成二级结构.f-9可以剪切dna序列，底物识别区域actg为保守序列.同时，对dna酶的剪切位点作了进一步研究，证明f-9是一个多位点剪切的dna酶.图6表1参19
| [1] | breakerrr,joycegf.adnaenzymethatcleavesrna[j].chembiol,1994,1(4):223-229
|
| [2] | breakerrr,joycegf.adnaenzymewithmg2+dependentrnaphosphoesteraseactivity[j].chembiol,1995,2(10):655-660
|
| [3] | faulhammerd,famulokm.theca2+ionasacofactorforanovelrnacleavingdeoxyribozyme[j].angewchemintedengl,1996,35(5):2837-2841
|
| [4] | faulhammerd,famulokm.characterizationanddivalentmetal-iondependentofinvitroselecteddeoxyribozymewhichcleavedna/rnachimericoligonucleotides[j].jmolbiol,1997,269(2):188-202
|
| [5] | santorosw,joycegf.ageneral-purposerna-cleavingdnaenzyme[j].procnatlacadsciusa,1997,94(9):4262-4266
|
| [6] | rotha,breakerrr.anaminoacidasacofactorforacatalyticpolynucleotide[j].procnatlacadsciusa,1998,95(11):6027-6031
|
| [7] | geyercr,send.evidenceforthemetal-cofactorindependenceofanrnaphosphodiester-cleavingdnaenzyme[j].chembiol,1997,4(8):579-593
|
| [8] | liy,breakerrr.phosphorylationdnawithdna[j].procnatlacadsciusa,1999,96(6):2746-2751
|
| [9] | liy,liuy,breakerrr.cappingdnawithdna[j].biochemistry,2000,39(11):3106-3114
|
| [10] | liy,geyerr,send.towardsanefficientdnazyme[j].biochemistry,1997,36:5589-5599
|
| [11] | carmin,balkhisr,breakerrr.cleavingdnawithdna[j].biochemistry,1998,95:2233-22237
|
| [12] | chandram,sachdevaa,silvermansk.dna-catalyzedsequence-specifichydrolysisofdna[j].natchembiol,2009,5:718-720
|
| [13] | dokukinv,silvermansk.lanthanideionsasrequiredcofactorsfordnacatalysts[j].chemsci,2012,3:1701-1714
|
| [14] | khachigianlm.catalyticdnasaspotentialtherapeuticagentsandsequence-specificmoleculartoolstodissectbiologicalfunction[j].jclininvest,2000,106(10):1189-1195
|
| [15] | chakrabortis,banerjeaac.identificationofcleavagesitesinthehiv-1tarrnaby10-23and8-17catalyticcontainingdnaenzymes[j].biomacromolecules,2003,4(3):568-571
|
| [16] | unwallah,banerjeaac.novelmono-anddi-dna-enzymestargetedtocleavetatortat-revrnainhibithiv-1geneexpression[j].antiviralres,2001,51(2):127-139
|
| [17] | wengelj,vesterb.lnaandalpha-l-lnatowardstherapeuticapplication[j].nucleosidesnucleotidesnucleicacid,2003,22(5):601-604
|
| [18] | 于乐成,王升启,顾长海,陈忠斌,毛青,刘鸿凌.脱氧核酶在转基因肝癌细胞中对hcv5’-非编码区的抑制活性[j].中华传染病杂志,2004,22(2):82-85[yuyc,wangsq,guch,chenzb,mzoq,liuhl.inhibitionofhcv5’-ncrbyspecificdeoxyribozumesintransgenehepg2cells[j].chinjinfectdis,2004,22(2):82-
|
| [19] | warashinam,kuwabarat,nakamatsuy,tairak.extremelyhighandspecificactivityofdnaenzymesincellwithphiladelphiachromosome[j].chembiol,1999,6(4):237-250
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
