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OALib Journal期刊
ISSN: 2333-9721
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东亚飞蝗prolixicin基因的克隆与表达分析
DOI: 10.3724/SP.J.1145.2013.00236, PP. 236-240
Keywords: 东亚飞蝗,抗菌肽,prolixicin,实时定量pcr,表达分析
Abstract:
猎蝽素（prolixicin）是最先发现于长红猎蝽rhodniusprolixus中的一种抗菌肽.本研究通过分析东亚飞蝗[locustamigratoriamanilensis(meyen)]转录组数据库，获得东亚飞蝗prolixicin基因的cdna序列，命名为lmprox.lmprox全长394bp，包含一个282bp的完整开放阅读框，编码93个氨基酸，理论分子量（mr）为9.97×103.氨基酸序列的n端有一个信号肽，c端有一个富含甘氨酸的g结构域.同源性分析表明lmprox与长红猎蝽的prolixicin同源性最高，一致性为48%，相似性为60%.进化关系分析表明，lmprox与长红猎蝽prolixicin的亲缘关系最近.基因定量分析显示，大肠杆菌刺激后2h，lmprox的表达明显上调，至8h表达量达到最高，随后开始缓慢下降，但仍然维持较高的表达水平，直到72h还显著高于对照.在未经大肠杆菌诱导的东亚飞蝗中，lmprox在脂肪体中的表达水平最高，其次是中肠，在头部和体壁中的表达水平较低；在诱导后的东亚飞蝗中，脂肪体和中肠中的lmprox表达显著增高，而头部和体壁中的表达水平没有变化.研究结果显示lmprox基因在大肠杆菌侵染后，迅速表达，且能持续较长时间的高水平表达，说明lmprox基因在东亚飞蝗的免疫防御过程中起着重要作用；脂肪体和中肠是lmprox基因主要诱导表达的器官.图5表1参20
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