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OALib Journal期刊
ISSN: 2333-9721
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甘薯sporamin家族基因克隆与表达分析
DOI: 10.3724/SP.J.1145.2013.00215, PP. 215-223
Keywords: 甘薯,储藏蛋白,基因克隆,序列分析,数字基因表达谱,蛋白酶抑制剂
Abstract:
为研究甘薯sporamin家族基因的结构和功能，通过对本实验室构建的甘薯转录组序列进行分析，设计相应引物并采用高保真dna聚合酶进行pcr扩增，经ta克隆和序列测定，利用在线软件对甘薯sporamin结构进行分析和功能预测.从转录组数据库中共发现61条sporamincontigs，其中sporamina家族有31条，sporaminb家族有30条，已从甘薯中克隆到6个sporamina基因和10个sporaminb基因.所有sporamina基因编码区全长660bp，基因间序列一致性为95%以上；sporaminb基因编码区全长651bp，基因间序列一致性达到98%；两个基因家族间序列一致性为84%.对克隆的sporamina和sporaminb进行的数字表达谱分析结果表明，sporamina和b在膨大初期的块根和成熟块根中的转录水平最高，在膨大中期转录水平较低，在叶片和茎中表达量极低.sporamin氨基酸保守序列和同源比对结果显示，该蛋白质含有sti（soybeantrypsininhibitor）保守序列，此序列有170多个氨基酸，属于kunitz型蛋白酶抑制剂超家族，表明该蛋白具有蛋白酶抑制剂功能.图9表3参33
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