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OALib Journal期刊
ISSN: 2333-9721
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水稻延伸蛋白基因osexpb7干涉载体的构建、遗传转化与基因功能分析
DOI: 10.3724/SP.J.1145.2013.00198, PP. 198-204
Keywords: 水稻,延伸蛋白,rnai干扰,农杆菌介导转化,植物激素
Abstract:
延伸蛋白（expansin）是植物细胞壁的重要组成成分，参与植物各种生理活动.利用rna干涉技术构建水稻osexpb7基因rnai干涉载体，通过农杆菌介导遗传转化将其导入粳稻品种日本晴.对转基因植株的定量pcr分析表明，转基因植株中osexpb7的表达量明显降低.与野生型日本晴相比，osexpb7下调t0代转基因植株株高变矮，秕谷率增高，谷粒变小.t1代转基因幼苗实验显示，与野生型相比，转基因植株生长缓慢，叶片披垂.在赤霉素诱导下转基因幼苗的生长抑制得到部分补偿，水淹条件下转基因植株的苗长伸长速度也慢于野生型，提示osexpb7基因可能通过调节细胞壁的延展而影响水稻生长发育，并受赤霉素的调控.图9表3参30
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