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OALib Journal期刊
ISSN: 2333-9721
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改进tail-pcr方法克隆产甲烷古菌的mcr基因簇
DOI: 10.3724/SP.J.1145.2013.00356, PP. 356-359
Keywords: tail-pcr,ad引物设计,甲基辅酶m还原酶,产甲烷古菌,mcr基因簇
Abstract:
甲基辅酶m还原酶（mcr）在产甲烷古菌中普遍存在并具有重要意义，其基因成簇存在，约5.8kb，其中α亚基核酸序列相对保守，是较易获得的保守序列.为了快速和简便地获得mcr基因簇，本研究根据甲基辅酶m还原酶β亚基（mcrb）的蛋白基序和密码子表设计了4条ad引物，经过改良的tail-pcr发现其中3条ad引物都获得了大于3kb并且正确的片段.本研究所获得的基因可为甲基辅酶m还原酶的深入研究奠定基础，相较于传统的tail-pcr方法，本研究对其的改进拓宽了其在获得大片段基因簇及侧翼序列方面的适用性，并可为其他类似基因簇的获得提供参考.图5表2参19
| [1] | 梁成真,张锐,郭三堆.染色体步移技术研究进展[j].生物技术通报,2009,10:75-82[liangcz,zhangr,guosd.progressofchromosomewalking[j].biotechnolbull,2009,10:75-
|
| [2] | ochmanh,gerberas,hartldl.geneticapplicationsofaninversepolymerasechainreaction[j].genetics,1988,120(3):621
|
| [3] | trigliat,petersonmg,kempdj.aprocedureforinvitroamplificationofdnasegmentsthatlieoutsidetheboundariesofknownsequences[j].nucleicacidsres,1988,16(16):8186
|
| [4] | muellerpr,woldb.invivofootprintingofamusclespecificenhancerbyligationmediatedpcr[j].science,1989,246(4931):780
|
| [5] | rileyj,butlerr,ogilvied,finniearr,jennerd,powells,anandr,smithj,markhama.anovel,rapidmethodfortheisolationofterminalsequencesfromyeastartificialchromosome(yac)clones[j].nucleicacidsres,1990,18(10):2887-2890
|
| [6] | rosenthala,jonesd.genomicwalkingandsequencingbyoligo-cassettemediatedpolymerasechainreaction[j].nucleicacidsres,199018(10):3095
|
| [7] | jonesdh,winistorfersc.sequencespecificgenerationofadnapanhardlepermitspcramplicationofunknownflankingdna[j].nucleicacidsres,1992,20(3):595
|
| [8] | liuyg,whittierrf.thermalasymmetricinterlacedpcr:automatableamplificationandsequencingofinsertendfragmentsfromp1andyacclonesforchromosomewalking[j].genomics,1995,25(3):674-681
|
| [9] | oharao,doritrl,gilbertw.one-sidedpolymerasechainreaction:theamplificationofcdna[j].pnas,1989,86(15):5673
|
| [10] | wesleycs,benm,kreitmanm,hagagn,eaneswf.cloningregionsofthedrosophilagenomebymicrodissectionofpolytenechromosomednaandpcrwithnonspecificprimer[j].nucleicacidsres,1990,18(3):599-603
|
| [11] | wuc,zhus,simpsons,dejongpj,dop-vectorpcr:amethodforrapidisolationandsequencingofinsertterminifrompacclones[j].nucleicacidsres,199624(13):2614
|
| [12] | 李付鹏,伍宝朵,马朝芝,傅廷栋.基于pcr的染色体步移技术研究进展[j].中国生物工程杂志,2010,30(12):87-94[lifp,wubd,macz,futd.progressofchromosomewalkingbypcramplificationtechniques[j].chinabiotechnol,2010,30(12):87-
|
| [13] | huangh,wangg,zhaoy,ship,luoh,yaob.directandefficientcloningoffull-lengthgenesfromenvironmentaldnabyrt-qpcrandmodifiedtail-pcr[j].applmicrobiolbiotechnol,2010,87(3):1141-1149
|
| [14] | zhouj,huangh,mengk,ship,wangy,luoh,yangp,baiy,yaob.cloningofanewxylanasegenefromstreptomycessp.tn119usingamodifiedthermalasymmetricinterlaced-pcrspecificforgc-richgenesandbiochemicalcharacterization[j].applbiochembiotechnol,2010,160(5):1277-1292
|
| [15] | ermleru,grabarsew,shimas,goubeaudm,thauerrk.crystalstructureofmethyl-coenzymemreductase:thekeyenzymeofbiologicalmethaneformation[j].science,1997,278:1457
|
| [16] | ellefsonw,wolfer.componentcofthemethylreductasesystemofmethanobacterium[j].jbiolchem,1981256:4259-4262
|
| [17] | halesba,edwardsc,ritchieda,hallg,pickuprw,saundersjr.isolationandidentificationofmethanogen-specificdnafromblanketbogpeatbypcramplificationandsequenceanalysis[j].applenvironmicrobiol,1996,62(2):668
|
| [18] | 仇艳光,田景汉,葛荣朝,赵宝存,沈银柱,黄占景.tail-pcr的改良及其在分离小麦基因启动子中的应用[j].生物工程学报,2008,24(4):695-699[qiuyg,tianjh,gerc,zhaobc,shenyz,huangzj.amodifiedtail-pcranditsapplicationinisolationgenepromoterofwheat[j].chinjbiotechnol,2008,24(4):695-
|
| [19] | liuyg,chenyl.high-efficiencythermalasymmetricinterlacedpcrforamplificationofunknownflankingsequences[j].biotechniques,2007,43:649-656
|
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