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OALib Journal期刊
ISSN: 2333-9721
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陶厄氏菌thauerasp.straintn9的鉴定及特性
DOI: 10.3724/SP.J.1145.2013.00318, PP. 318-323
Keywords: thauera,nirs,nosz,胞外多聚物,生物脱氮,废水处理
Abstract:
菌株tn9由耐受高碳氮负荷的好氧颗粒污泥中筛选获得，对其进行了初步鉴定、脱氮功能基因及胞外多聚物（eps）方面的研究.根据16srdna序列确定菌株tn9的分类地位；通过pcr检测其脱氮相关基因并测序；采用高速离心-乙醇低温沉淀法提取eps，分别测定了蛋白质和多糖的含量.结果表明：tn9为陶厄氏菌属（thauera）细菌.检测获得了nirs、nosz基因序列，其中该菌株的nirs与thauerasp.27的相似性为91%，nosz与thauerasp.mz1t的相似性为95%.但是16srdna和nirs基因、16srdna和nosz基因之间的系统进化相关性较小.该菌株能够产生eps，72h培养物中eps的蛋白质含量为44.4-50.7mg/g(ts)，多糖含量为16.8-18.6mg/g(ts)，蛋白质和多糖的含量比为2.4-3.0.因此，菌株tn9是一株具有脱氮和eps分泌能力的陶厄氏菌，在废水处理方面具有应用潜力.图4表1参28
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