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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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白腐真菌对异狄氏剂的降解特性及降解机理
DOI: 10.3724/SP.J.1145.2013.00119, PP. 119-125
Keywords: 白腐真菌,异狄氏剂,生物降解,降解特性,代谢产物
Abstract:
异狄氏剂作为一种防治农业害虫的杀虫剂在世界范围内曾被大量使用，其在环境中的残留及生物毒性已经引起广泛关注.为开发异狄氏剂降解菌资源，并阐明异狄氏剂的微生物降解机制，采用液体培养法研究了一株对狄氏剂具有降解能力的白腐真菌phlebiaacanthocystistmic34875对异狄氏剂的降解性能及机理.结果表明，在菌体接种量为15%（v/v）时，异狄氏剂在10d培养期间的降解率达到最高的57%左右.该菌株在异狄氏剂的初始浓度为20μmoll-1时具有最大降解速率，为0.053μmoll-1h-1.在培养温度为25-35℃，ph值为4.5-5.5之间时，菌株对异狄氏剂具有较好的降解效果.相同条件下，菌株在马铃薯液体培养基中显示出比在kirk合成液体培养基中更高的降解活性.通过气相色谱？质谱分析发现了多个未曾报道过的代谢产物，包括3个单一羟基化产物和1个羧基化产物，表明该菌株对异狄氏剂的降解途径不同于已知的细菌降解途径.
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