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OALib Journal期刊
ISSN: 2333-9721
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克雷伯氏菌产1,3-丙二醇菌株选育及甘油脱水酶基因转录分析
DOI: 10.3724/SP.J.1145.2013.00025, PP. 25-29
Keywords: 克雷伯氏菌,1,3-丙二醇,紫外诱变,甘油脱水酶,半定量rt-pcr
Abstract:
通过对野生克雷伯氏菌（klebsiella）进行紫外诱变，获得一株耐高浓度甘油及高浓度1,3-丙二醇的突变菌株a-39-3，该突变株产1,3-丙二醇的量较野生菌株提高了58%，副产物2,3-丁二醇和乙醇的量分别降低了27%和19%.为探讨突变株高产1,3-丙二醇的原因，通过对突变菌株发酵过程中关键酶的酶活跟踪分析，发现1,3-丙二醇氧化还原酶（pdor）的酶活力与野生菌株相差不大，甘油脱氢酶（gdh）的酶活力略有下降，而突变菌株的甘油脱水酶（gdht）的酶活力明显高于野生菌株.并采用半定量rt-pcr方法，从转录水平上比较甘油脱水酶基因的差异，结果发现突变菌株a-39-3甘油脱水酶基因的相对mrna转录水平是野生菌株k的2.58倍，分析说明该突变株1,3-丙二醇产量的提高与关键酶基因dhab的mrna转录水平关系密切.
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