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OALib Journal期刊
ISSN: 2333-9721
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土生空团菌培养基优化与摇瓶培养
DOI: 10.3724/SP.J.1145.2013.00152, PP. 152-156
Keywords: 土生空团菌,正交试验,培养基,优化,液体发酵
Abstract:
在单因素试验的基础上，通过正交试验对cgso1、cgsb2、cgo5和spop2这4株土生空团菌[cenococcumgeophilumfr.(cg)]的培养基配方分别进行了优化.结果发现，来源不同的cg菌株对培养基组分需求存在一定差异，碳氮源、微量元素、无机盐以及维生素都不相同，cgso1、cgsb2、cgo5对葡萄糖和麦芽汁的混合c源以及牛肉浸膏利用最好，spop2对麦芽汁以及酒石酸铵的利用最好，适量的微量元素、无机盐以及维生素b1会促进cg的生长.经平板培养基优化后的培养基配方可应用到液体培养中，这4株cg菌经液体培养后菌丝产量分别达到了13.2、12.4、18.9和15.1g/l，均可满足液体发酵的生产要求.
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