|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
拟南芥蛋白磷酸酶pph1的结构预测与功能分析
DOI: 10.3724/SP.J.1145.2013.00069, PP. 69-73
Keywords: pph1,去磷酸化,pp2c,核心结构域,抗体
Abstract:
拟南芥pph1属于蛋白磷酸酶2c（pp2c）家族，主要参与捕光色素复合物lhcⅱ蛋白去磷酸化调控与光系统的状态转移，与stn7蛋白激酶共同参与调控lhcⅱ的磷酸化与去磷酸化.为了解此酶的具体的催化机制和空间结构，使用生物信息学的方法对pph1的疏水性、跨膜区、膜体和二级结构进行分析，构建系统进化树并通过同源建模的方法建立其核心结构域的三级结构，围绕蛋白磷酸酶的结构和可能的作用机制关系进行探讨.在以上分析的基础上选择特异位点合成多肽后，免疫家兔并成功制备抗体.蛋白印迹结果显示制备得到了pph1特异抗体，证实此磷酸酶结构得以正确地预测.研究为今后进一步研究其结构和功能以及突变体的设计提供了理论基础.
| [1] | 1allenjf.proteinphosphorylationinregulationofphotosynthesis[j].biochimbiophysacta,1992,1098:275-335
|
| [2] | 2bellafiores,barnechef,peltierg,rochaixjd.statetransitionsandlightadaptationrequirechloroplastthylakoidproteinkinasestn7[j].nature,2005,433:892-895
|
| [3] | 3shapiguzova,ingelssonb,samoli,andresc,kesslerf,rochaixjd,venerav,goldschmidtcm.thepph1phosphataseisspecificallyinvolvedinlhciidephosphorylationandstatetransitionsinarabidopsis[j].procnatlacadsciusa,2010,107:4782-4787
|
| [4] | 4pribilm,pesaresip,hertlea,barbator,leisterd.roleofplastidproteinphosphatasetap38inlhciidephosphorylationandthylakoidelectronflow[j].plosbiol,2010,8:1-12
|
| [5] | 5gattikera,gasteigere,bairocha.scanprosite:areferenceimplementationofaprositescanningtool[j].applbioinform,2002,1:107-108
|
| [6] | 6higginsdg,thompsonjd,gibsontj.usingclustalformultiplesequencealignments[j].methenzymol,1996,266:383-402
|
| [7] | 7kumars,tamurak,neim.mega3:integratedsoftwareformolecularevolutionarygeneticsanalysisandsequencealignment[j].briefbioinform,2004,5:150-163
|
| [8] | 8李佳,郑清川,杜林方.烟草病程相关蛋白pr-1a的三维模型和荧光光谱[j].应用与环境生物学报,2008,14(4):466~468[lij,zhengqc,dulf.homologymodelingandfluorescencespectrastudyoftobaccopr-1a[j].chinjapplenvironbiol,2008,14(4):466~468
|
| [9] | 9kelleyla,maccallumrm,sternbergmje.enhancedgenomeannotationusingstructuralprofilesintheprogram3d-pssm[j].jmolbiol,2000,299(2):499-520
|
| [10] | 10saylera,milner-whiteej.rasmol:biomoleculargraphicsforall[j].trendsbiochemsci,1995,20:374-376
|
| [11] | 11李晓鹏,杜林方,梁厚果,吴宛荪.psⅱ反应中心d1蛋白的小肽抗体的制备和鉴定[j].生物化学与生物物理进展,1997,24(3):283~285[lixp,dulf,lianghg,wuws.preparationandidentificationofantidodecapeptideofpolypeptided1ofphotosystemⅱreactioncenter[j].progbiochembiophys,1997,24(3):283~
|
| [12] | 12weihm,guojw,zhangs,huangb,dulf.thepresenceofphosphorylationformofd1proteininitscross-linkedaggregatesinhighlighttreatedspinachleavesinvivo[j].chinscibull,2006,51:69-74
|
| [13] | 13dulf,zhanglx,lianghg.preliminaryanalysisofproteasesassociatedwithphotosystemⅱparticles[j].chinscibull,1993,38:1903-1907
|
| [14] | 14ilonaf,viktord,martinm,laszlo´s,csabak.arabidopsispppfamilyofserine/threoninephosphatases[j].trendsplantsci,2007,12:169-176
|
| [15] | 15shiyg.serine/threoninephosphatases:mechanismthroughstructure[j].cell,2009,139:468-484
|
| [16] | 16bennettj.phosphorylationofchloroplastmembranepolypeptides[j].nature,1977,269:344
|
| [17] | 17dasak,helpsnr,cohenpt,barfordd.crystalstructureoftheproteinserine/threoninephosphatase2cat2.0åresolution[j].emboj,1996,15:6798-6804
|
| [18] | 18胡学博,宋凤鸣,郑重.高等植物中蛋白磷酸酶2c的结构与功能[j].细胞生物学杂志,2005,27(1):29~34[huxb,songfm,zhengz.thestructureandfunctionofproteinphosphatase2csinhigherplants[j].chinjcellbiol,2005,27(1):29~
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
