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OALib Journal期刊
ISSN: 2333-9721
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电活性生物膜：形成、表征及应用
DOI: 10.3724/SP.J.1145.2014.03028, PP. 1096-1103
Keywords: 电活性生物膜,电活性微生物,胞外电子传递,生物电化学系统,微生物电合成
Abstract:
电活性生物膜（electrochemicallyactivebiofilms，eabs）是一类能够直接与胞外固态载体（铁氧化物、腐殖质及电极等）进行电子交换的生物膜.eabs的电子传递特性，赋予了它在环境、能源和化工等领域的广泛应用前景，已成为当前国际研究热点.本文以革兰氏染色法为依据，分别介绍了腐败希瓦氏菌（shewanellaputrefaciens）、硫还原地杆菌（geobactersulfurreducens）和丁酸梭菌（clostridiumbutyricumeg3）为代表的阴性和阳性电活性微生物；在普通生物膜的形成基础上，讨论了eabs的两种主要培养方法；分别从eabs输出电子与接受电子的角度，详细论述了电活性微生物与胞外载体的电子传递机制；重点阐述了利用电化学、光谱学、电子显微镜、分子生态学等多技术手段表征单个电活性微生物和整个eabs的形态、结构，以及所揭示的胞外电子传递机制和相关影响因子；对eabs在电能输出、污染物治理、有价品合成等方面应用作了详细介绍.最后，建议对eabs的研究建立一个统一、标准的表征方法，同时应重点研究eabs接受电子的传递机制.对这些机理的深入了解，可使得eabs在污染物治理以及有机物的电合成等方面应用早日实现规模化、产业化生产.
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