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OALib Journal期刊
ISSN: 2333-9721
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海洋沉积物中六溴环十二烷的微生物降解特性及降解菌的分离鉴定
DOI: 10.3724/SP.J.1145.2014.03019, PP. 1070-1075
Keywords: hbcds,海洋沉积物,微生物降解,降解菌株
Abstract:
为了解六溴环十二烷（hbcds）在海洋沉积物中的好氧微生物降解特性，在以hbcds为唯一碳源的无机培养基中添加海洋沉积物悬浮液，利用hplc-ms/ms测定hbcds降解率，并驯化分离得到hbcds降解菌株，进行菌种鉴定.结果显示：海洋沉积物好氧微生物对hbcds的降解半衰期为38-61d；在120d中hbcds共降解了72%；从海洋沉积物中富集、分离得到5株能够以hbcds为唯一碳源的降解菌株，分别命名为ctf711-a、ctf711-c、ctf711-d、ctf711-e和ctf711-x，结合形态学观察、生理生化试验和16srdna序列分析，将其依次鉴别为假单胞菌属、红球菌属、不动杆菌属、微杆菌属和盐单胞菌属，除假单胞菌属外，其余菌株对hbcds的降解能力还尚未见报道.同时将这5株菌分别培养与混合培养，混合菌株对hbcds降解率最高.本研究报道了5株不同属的hbcds好氧降解菌株，且混合菌株对hbcds降解效果较好，结果可为hbcds的生物修复提供理论和应用依据.
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