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OALib Journal期刊
ISSN: 2333-9721
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0#柴油和原油水溶性成分对黑鲷肝脏7-乙氧基异吩？唑酮-脱乙基酶活性及其mrna表达的影响
DOI: 10.3724/SP.J.1145.2014.05029, PP. 1027-1032
Keywords: 黑鲷,0#柴油,原油,水溶性成分,7-乙氧基异吩？唑酮-脱乙基酶,细胞色素p4501a1mrna
Abstract:
燃料油和原油是海洋环境中石油污染的主要类型，其对海洋生物的毒性作用是评价海洋环境质量的基础，为了解两种石油污染在同一环境条件下对同一海洋生物的毒性差异，研究了0#柴油和原油水溶性成分（wateraccommodatedfraction，waf）对黑鲷（sparusmacrocephlus）肝脏7-乙氧基异吩？唑酮-脱乙基酶（erod）活性及细胞色素p4501a1(cyp1a1)mrna表达量的影响.结果显示，在富集阶段（15d），第1天0#柴油和原油waf0.06mg/l和0.03mg/l实验组肝脏的erod活性、cyp1a1mrna表达量均显著增加（p<0.05），随后0#柴油和原油waf两个浓度实验组肝脏的erod活性均继续上升，分别在第5天、第10天达到峰值，随后下降；0#柴油waf两个浓度实验组肝脏的cyp1a1mrna表达量在第1天后即开始下降，原油waf两个浓度实验组肝脏的cyp1a1mrna表达量在第5天达到峰值后开始下降.在释放阶段（10d）结束后，除了原油waf0.03mg/l实验组外，0#柴油waf的两个浓度实验组和原油waf0.06mg/l实验组肝脏的erod活性、cyp1a1mrna表达量均仍然显著高于对照组水平（p<0.05）.本研究表明，两种石油污染物waf对黑鲷肝脏的erod活性、cyp1a1mrna诱导存在差异，0#柴油waf实验组erod活性、cyp1a1mrna表达量均高于原油waf实验组，且0#柴油waf实验组erod活性、cyp1a1mrna表达量下降时间早于原油实验组，原油实验条件下黑鲷肝脏的erod活性、cyp1a1mrna表达量的恢复能力高于0#柴油.
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