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OALib Journal期刊
ISSN: 2333-9721
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同型产乙酸菌富集物的群落解析及转化合成气产乙酸
DOI: 10.3724/SP.J.1145.2014.04040, PP. 1052-1057
Keywords: 同型产乙酸菌,合成气,富集,产乙酸,微生物群落,clostridium,挥发性脂肪酸
Abstract:
合成气生物转化是一项具有巨大应用前景的技术，对大力开发可再生资源和促进国家可持续发展具有重要意义.本研究采集牛粪样品进行同型产乙酸菌富集培养，利用富集物分别在中温（37℃）和高温（55℃）条件下生物转化h2/co2气体，通过克隆文库技术分析中温和高温富集物的微生物群落组成，gc气相色谱法检测气体转化产生的挥发性脂肪酸（vfas）浓度.结果表明，牛粪样品中温富集物和高温富集物同型产乙酸菌数量分别增长了77倍和189倍，富集物中同型产乙酸菌分布于clostridium、oxobacter、caloramator、thermoanaerobacter及moorella属，其中clostridium属最多.同型产乙酸菌富集物在中温和高温条件下利用h2/co2气体生成的最高乙酸浓度分别为43和38mmol/l.该富集物可稳定、持续、有效地生物转化h2/co2气体产乙酸，并且偏碱性、中温条件有利于提高转化效率.本文研究表明这种区别于传统纯培养法的混合培养技术能够达到产乙酸的目的，可为合成气生物转化提供新思路.
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