|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
过量表达沙冬青巯基蛋白酶抑制剂基因ampi提高大肠杆菌低温与热胁迫抗性
DOI: 10.3724/SP.J.1145.2010.00341, PP. 341-346
Keywords: 沙冬青,ampi,大肠杆菌,融合蛋白,过量表达,非生物胁迫
Abstract:
沙冬青是我国北方内蒙古荒漠地区特有的常绿阔叶灌木，具有很强的抗低温、干旱、盐碱能力，是重要的抗逆基因资源植物.将从低温诱导的沙冬青幼苗中克隆获得的巯基蛋白酶抑制剂基因ampi亚克隆后构建到原核表达载体ptwin1上，得到重组表达载体pt-pi，双酶切及测序结果证明pt-pi已经构建成功.将pt-pi转入大肠杆菌er2566中，经异丙基β-硫代半乳糖苷（iptg）诱导后，sds-page电泳检测发现有mr35×103的目的融合蛋白表达，与预测结果一致，其中以在37℃诱导3~4h条件下表达量最高，表明目的基因ampi在大肠杆菌中得到了成功表达.分别测定了低温（0℃）和热胁迫（50℃）条件下ampi宿主菌的生存能力，结果表明ampi的表达能提高宿主菌的存活率，可能是ampi对细胞具有保护功能.图9参28
| [1] | 8huangy,xiaob,xiongl.characterizationofastressresponsiveproteinaseinhibitorgenewithpositiveeffectinimprovingdroughtresistanceinrice.planta,2007,226:73~85
|
| [2] | 9dombrowskije.saltstressactivationofwound-relatedgenesintomatoplants.plantphysiol,2003,132:2098~2107
|
| [3] | 13yangzh(杨朝辉),leijj(雷建军),caobh(曹必好),songm(宋明),wangyp(王亚培),wangj(王进).cloningofcowpeatrypsinproteinaseinhibitorgene.actabotboroccidsin(西北植物学报),2002,22(5):1050~1055
|
| [4] | 14delledonnem,allegrog,belenghib,balestrazzia,piccof,levinea,zelascos,calligarip,confalonierim.transformationofwhitepoplar(populusalbal.)withanovelarabidopsisthalianacysteineproteinaseinhibitorandanalysisofinsectpestresistance.molbreed,2001,7:35~42
|
| [5] | 15kimjg,baeksa,imkh.overexpressionofakunitz-typetrypsininhibitor(atkti1)causesearlyfloweringinarabidopsis.plantgrowthregul,2009,59:75~81
|
| [6] | 16liumq(刘美芹),lucf(卢存福),yinwl(尹伟伦).researchprogressofbiologicalcharacteristicsandabioticstresstolerantmechanismsofrareandendangeredplantammopiptanthus.chinjapplenvironbiol(应用与环境生物学报),2004,10(3):384~38
|
| [7] | 17liumq,lucf,shenx,yinwl.characterizationandfunctionanalysisofacold-inducedamcipgeneencodingadehydrin-likeproteininammopiptanthusmongolicus.dnaseq,2006,17:342~349
|
| [8] | 18liumq,chenyy,lucf,zhangh,yinwl.coldacclimationinducedaccumulationofphenoliccompoundsandfreezingtoleranceinammopiptanthusmongolicus.forstudieschina,2007,9(3):203~207
|
| [9] | 19liumq,shenx,yinwl,lucf.functionalanalysisofcold-induciblecdnaclonesinthelegumeammpiptanthusmongolicus.cryoletters,2005,26:213~222
|
| [10] | 25wagnerma,zahrld,rieserge,andkoraimanng.growthphase-andcelldivision-dependentactivationandinactivationoftheσ32reguloninescherichiacoli.jbacteriol,2009,191(5):1695~1702
|
| [11] | 26zhangxx,liusk,takanot.twocysteineproteinaseinhibitorsfromarabidopsisthaliana,atcysaandatcysb,increasingthesalt,drought,oxidationandcoldtolerance.plantmolbiol,2008,68:131~143
|
| [12] | 27casatip,drincovichmf,edwardsge,andreocs.malatemetabolismbynadp-malicenzymeinplantdefense.photosynthres,1999,61:99~105
|
| [13] | 28luzq,liud,liusk.tworicecytosolicascorbateperoxidasesdifferentiallyimprovesalttoleranceintransgenicarabidopsis.plantcellrep,2007,26:1909~1917
|
| [14] | 1ryanca.proteaseinhibitorsinplants:genesforimprovingdefensesagainstinsectsandpathogens.annurevphytopathol,1990,28:425~449
|
| [15] | 2chengzy(程仲毅),xueqz(薛庆中).thestructureandregulationofplantproteinaseinhibitorgenesandtheirstrategyinpestcontrol.actagensin(遗传学报),2003,30(8):790~796
|
| [16] | 3yanggd(杨广东),zhuz(朱祯),liye(李燕娥),zhuzj(朱祝军),shangguanxx(上官晓霞).obtaininginsectresistancetransgenicsweetpeppertransformedwithmodifiedcptigene(sck).chinjapplenvironbiol(应用与环境生物学报),2002,8(3):239~244
|
| [17] | 4pernasm,sanchez-monger,gomezl,salcedog.achestnutseedcystatindifferentiallyeffectiveagainstcysteineproteinasesfromcloselyrelatedpests.plantmolbiol,1998,38:1235~1242
|
| [18] | 5yangah,yehkw.molecularcloning,recombinantgeneexpression,andantifungalactivityofcystatinfromtaro(colocasiaesculentacv.kaosiungno.1).planta,2005,221:493~501
|
| [19] | 6belenghib,acconciaf,trovatom,perazzollim,bocedia,poiticefif,ascenzip,delledonnem.atcys1,acystatinfromarabidopsisthaliana,suppresseshypersensitivecelldeath.eurjbiochem,2003,270(12):593~604
|
| [20] | 7vyvercv,schneidereitj,driscolls,turnerj,kunertk,foyerch.oryzacystatiniexpressionintransformedtobaccoproducesaconditionalgrowthphenotypeandenhanceschillingtolerance.plantbiotechj,2003,1:101~112
|
| [21] | 10chenj,liuj,guol,zhailj,chenzl,guhy.inducibleexpressionpatternofricebowman-birkinhibitorgeneoswip1-2anditsproteaseinhibitoryactivity.chinscibull,2004,49(7):657~661
|
| [22] | 11solomonm,belenghib,delledonnem,menacheme,levinea.theinvolvementofcysteineproteasesandproteaseinhibitorgenesintheregulationofprogrammedcelldeathinplants.plantcell,1999,11:431~443
|
| [23] | 12zhangz,liyy,lic,yuanjm,wanzh.expressionofabuckwheattrypsininhibitorgeneinescherichiacolianditseffectonmultiplemyelomaim-9cellproliferation.actabiochimbiophyssin,2007,39(9):701~707
|
| [24] | 20sotoa,allonai,colladac,guevarama,casador,aragoncilloc,gomezl.heterologousexpressionofaplantsmallheat-shockproteinenhancesescherichiacoliviabilityunderheatandcoldstress,plantphysiol,1999,120:521~528
|
| [25] | 21wangzx(王朝霞),jiangcj(江昌俊),yuyb(余有本),fangwp(房婉平).expressionoftheteacystatincdnaine.coli.actalaserbiolsin(激光生物学报),2006,15(6):608~612
|
| [26] | 22lany,caid,zhengyz.expressioninescherichiacoliofthreedifferentsoybeanlateembryogenesisabundant(lea)genestoinvestigateenhancedstresstolerance.jintegrplantbiol,2005,47:539~603
|
| [27] | 23strocchim,ferrerm,timmiskn,golyshinpn.lowtemperature-inducedsystemsfailureinescherichiacoli:insightsfromrescuebycold-adaptedchaperones.proteomics,2006,6:193~206
|
| [28] | 24arsenef,tomoyasut,bukaub.theheatshockresponseofescherichiacoli.internjfoodmicrobiol,2000,55:3~9
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
