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OALib Journal期刊
ISSN: 2333-9721
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一株轻度嗜盐反硝化菌的分离鉴定及特性
DOI: 10.3724/SP.J.1145.2010.00394, PP. 394-398
Keywords: 嗜盐菌,好氧反硝化菌,系统发育分析,高盐废水,生物降解
Abstract:
从处理高盐度废水的成熟活性污泥中分离筛选得到1株轻度嗜盐反硝化细菌yl-1.通过对该菌株的形态观察、生理生化实验以及16srdna基因序列分析，确定该菌株为迪茨氏菌（dietziasp.）.盐度影响实验表明，该菌株能在盐度为0~10%的培养液中生长，盐度为3%时d600nm达到最大，为2.002；当初始硝态氮浓度为120mg/l左右时，do值对该菌株的反硝化效果几乎没有影响，nox--n去除率均在90%以上，表明该菌为好氧反硝化菌；适于在碱性环境中生长，最适ph为7.5~8.5；能利用乙酸钠、蔗糖、葡萄糖、柠檬酸钠、丁二酸钠为碳源进行反硝化，以蔗糖为碳源时反硝化效果最好，nox--n去除率达到99.8%；在25~30℃的温度范围内反硝化效果较好，30℃时菌株的nox--n去除率超过90%.该菌在一定盐度下具有较好的反硝化作用，能为高盐度废水的生物处理提供一定的参考价值.图7参15
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