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OALib Journal期刊
ISSN: 2333-9721
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纤维素酶产生菌hs-f9的筛选鉴定和产酶条件优化
DOI: 10.3724/SP.J.1145.2010.00274, PP. 274-278
Keywords: 纤维素酶,菌株筛选,产酶条件优化,酶活性
Abstract:
为了选育高产纤维素酶菌株，通过刚果红鉴别培养基以及滤纸条崩解实验测定，从牛粪堆肥中筛选到一株产纤维素酶的真菌hs-f9，根据菌株形态特性和18srrna基因序列分析，初步鉴定该菌为绿色木霉（trichodermaviride）.利用液体发酵培养产生纤维素酶，研究了碳源、氮源、培养时间、培养温度、培养基起始ph、接种量对菌株hs-f9产酶的影响.结果表明：产eg、cbh和fpa的最适碳源均为cmc-na；eg和cbh在以蛋白胨为唯一氮源时酶活最高，fpase则在以黄豆粉为唯一氮源时酶活最高；产生eg、cbh和fpa的最适温度分别为30℃、30℃、33℃；最适起始ph为3.0、3.0和4.0；eg和fpase的最适接种量为2%，cbh最适接种量达到了8%；培养时间均以5~6d为宜.在最适条件下培养，该菌株eg、cbh和fpase的酶活分别达到了5275.3u/ml、8502.1u/ml和3619.1u/ml，是未优化前的1.42、1.35和1.32倍.图6表2参23
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