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OALib Journal期刊
ISSN: 2333-9721
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胞外初始底物浓度对产氢光合细菌葡萄糖跨膜传输及代谢的影响
DOI: 10.3724/SP.J.1145.2010.00264, PP. 264-268
Keywords: 产氢光合细菌,胞内葡萄糖浓度,葡萄糖传输速率,葡萄糖酶酵解速率,葡萄糖渗透系数
Abstract:
对产氢光合细菌葡萄糖跨膜传输速率、葡萄糖酶酵解速率进行了实验研究，对胞内葡萄糖浓度、葡萄糖传输渗透系数进行了计算.实验结果表明，胞外初始底物浓度为50~100mmol/l时，葡萄糖传输速率、胞内葡萄糖浓度总体都随胞外初始底物浓度的增大而增大，但胞外初始底物浓度增大至150mmol/l时，对葡萄糖跨膜传输产生了限制作用，葡萄糖传输速率和胞内葡萄糖浓度均相应减小；计算得到胞内葡萄糖浓度最小值为0.34mmol/l，最大为5.87mmol/l；初始底物浓度为15~35mmol/l时，细胞破碎液葡萄糖酶酵解速率随底物浓度的增大而增大；胞外初始底物浓度为50~150mmol/l时，葡萄糖代谢速率受葡萄糖传输速率控制，产氢光合细菌葡萄糖平均渗透系数为231.13cm3h-1g(dw)-1，葡萄糖传输速率主要受胞内外葡萄糖浓度势差控制；75mmol/l为较有利于葡萄糖跨膜传输的最佳胞外初始底物浓度.图5参11
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