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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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bngid1基因过量表达对甘蓝型油菜矮化突变体ndf-1株高的恢复
DOI: 10.3724/SP.J.1145.2010.00192, PP. 192-196
Keywords: 甘蓝型油菜,bngid1基因,矮化突变体,过量表达载体,遗传转化,株高恢复
Abstract:
在正常株高油菜中克隆了与拟南芥矮化突变相关基因atgid1同源的bngid1基因全长cdna目的片段，将其正向插入表达载体pfgc5941上的camv35s启动子下游，构成该基因过量表达载体pfgc5941-bngid1.经根癌农杆菌介导，导入甘蓝型油菜矮化突变体ndf-1基因组，通过pcr及半定量rt-pcr检测，确定得到抗性转基因植株23株.移至大田生长，观察结果显示，转基因植株明显高于对照矮化突变体ndf-1，平均高出49.05cm.结果表明，bngid1基因过量表达能够使甘蓝型油菜矮化突变体ndf-1株高得到较大程度的恢复.图7表2参16
| [1] | 1daviespjed.planthormones:physiology,biochemistryandmolecularbiology.dordrecht,thenetherlands:kluwer,1995.233~241
|
| [2] | 2tanx(谈心),maxr(马欣荣).advanceinresearchofgibberellinbiosynthesispathway.chinjapplenvironbiol(应用与环境生物学报),2008,14(4):571~577
|
| [3] | 3suntp,frankg.gibberellinsignaltransduction.curropinplantbiol,2000,3:374~380
|
| [4] | 4ueguchi-tanakam,ashikarim,nakajimam,itohh,katohe,kobayashim,chowty,hsingyc,kitanoh,yamaguchii,matsuokam.gibberellininsensitivedwarf1encodesasolublereceptorforgibberellin.nature,2005,437:693~698
|
| [5] | 5nakajimm,shimadaa,takashiy.identicationandcharacterizationofarabidopsisgibberellinreceptors.plantj,2006,46:880~889
|
| [6] | 7hironorii,makotom,camillem,stebercm.arolefortheubiquitin�26s-proteasomepathwayingibberellinssignaling.revtrendsplantsci,2003,8(10):492~497
|
| [7] | 8dilla,suntp.synergisticde-repressionofgibberellinssignalingbyremovingrgaandgaifunctioninarabidopsisthaliana.genetics,2001,159:777~785
|
| [8] | 6ueguchi-tanakam,nakajimam,motoyukia,matsuokam,gibberellinreceptoranditsroleingibberellinsignalinginplants.annu.plantbiol,2007,58:183~98
|
| [9] | 9wangml,zhaoy,chenf.inheritanceandpotentialsofamutateddwarfinggene‘ndf-1’inbrassicanapus.plantbreeding,2004,123(5):449~454
|
| [10] | 10gaoy(高勇),zhaoy(赵云),liuw(刘薇),zhangy(张宇),zhouzg(周增光),zhangzy(张珍勇),wangml(王茂林).effectsofexogenouslyappliedhormonesonseedlingelongationandcontentofendogenousga3inadwarfmutant(brassicanapusl.).jsichuanunivnatscied(四川大学学报自然科学版),2007,44(5):1133~1137
|
| [11] | 11lihp(李华鹏).mappingforadwarfmutatedsiteandcloningandanalysisofthegid1geneofbrassicanapusdwarfmutantndf-1:[doctordegree’sdissertation].chengdu,china:sichuanuniversity(成都:四川大学),2008
|
| [12] | 12zhangnh,weizq,hejx,dulf,lianghg.anefficientandeconomicmethodforpreparationofhighqualityplantrna.progrbiochem&biophys,2004,31(10):947~950
|
| [13] | 13rhodesca,pierceda,metllerj.geneticallytransformedmaizeplantsfromprotoplasts.science,1988,240:204~207
|
| [14] | 14zhanghy(张红岩).full-lengthcdnacloningofcysteineproteaseinbrassicanapusl.anditsantisensegenetransformation:[masterdegree’sthesis].chengdu,china:sichuanuniversity(成都:四川大学),2008
|
| [15] | 15griffithsj,murasek,rieui.geneticcharacterizationandfunctionalanalysisofthegid1gibberellinreceptorsinarabidopsis.plantcell,2006,18:3399~3414
|
| [16] | 16iuchis,suzukih,kimyc.multipleloss-offunctionofarabidopsisgibberellinreceptoratgid1scompletelyshutsdownagibberellinsignal.plantj,2007,50:958~966
|
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